Exploring the antibiofilm effect of nitrofurantoin and rutin in uropathogenic Klebsiella pneumoniae.

IF 2.6 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2025-08-01 Epub Date: 2025-07-17 DOI:10.1080/08927014.2025.2532675

Seetha Lakshmi Rajangam, Kakithakara Vajravelu Leela, Manonmoney Jayaraman, Manoj Kumar Narasimhan

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Abstract

Biofilm formation is a critical factor in uropathogenic Klebsiella pneumoniae, enabling colonization in the urinary tract and contributing to biofilm-mediated antibiotic resistance. This study evaluates the anti-biofilm and antimicrobial properties of the nitrofurantoin-rutin (Ntf-Rut) combination against a strong biofilm-forming clinical isolate, K. pneumoniae SLMK002, screened from Klebsiella spp. (n = 15) causing urinary tract infections (UTI). The findings showed Ntf-Rut combination significantly inhibited biofilm mass by 81%, with a 74% reduction in extracellular polymeric substances (EPS). Colony Forming Unit (CFU) enumeration analysis revealed higher cell death in mature biofilm, with CFU reduced from 9 ± 1 × 10⁶ to 2 ± 1 × 10⁶. Further, flow cytometry results prove that the combination induces cell membrane damage. In conclusion, the findings depict that the Ntf-Rut combination effectively reduces and inhibits the biofilm of K. pneumoniae SLMK002.

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探讨呋喃妥因和芦丁对尿路致病性肺炎克雷伯菌的抗菌作用。

生物膜的形成是尿路致病性肺炎克雷伯菌的关键因素，使其能够在尿路中定植，并促进生物膜介导的抗生素耐药性。本研究评估了呋喃妥英-芦丁（Ntf-Rut）联合药物对从导致尿路感染（UTI）的克雷伯氏菌（n = 15）中筛选的强生物膜形成临床分离物肺炎克雷伯氏菌SLMK002的抗生物膜和抗菌性能。结果表明，Ntf-Rut组合显著抑制生物膜质量81%，细胞外聚合物（EPS）减少74%。菌落形成单位（Colony Forming Unit， CFU）计数分析显示成熟生物膜细胞死亡率较高，CFU由9±1 × 106降至2±1 × 106。此外，流式细胞术结果证明，该组合诱导细胞膜损伤。综上所述，Ntf-Rut联合有效地降低和抑制了肺炎K. SLMK002的生物膜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.