Engineered Probiotics for Colitis Therapy by Improving Intestinal Colonization and Scavenging ROS.

IF 5.1 Q1 POLYMER SCIENCE

ACS Macro Letters Pub Date : 2025-07-17 DOI:10.1021/acsmacrolett.5c00295

Siyuan Yuan,Xiaomei Dai,Yuqin Zou,Menglin Huang,Xue Yang,Feng Gao

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引用次数: 0

Abstract

Probiotics hold significant promise for treating colitis, but their application remains challenging because of insufficient colonization and survival against colorectal oxidative stress. Herein, engineered probiotics were prepared to enhance probiotics' colonization and scavenge the overexpressed reaction oxygen species (ROS) at the site of colitis. First, primary amino groups on the Lactobacillus rhamnosus (LAB) surface were converted to free thiols by a simple one-step imidoester reaction. Then, we used thiol-initiated ring-opening cascade of dithiolanes to prepare a class of structurally dynamic microgels on the LAB surface (LAB-gel) by reacting alpha-lipoic acid (LA) and sodium thioctate (LANa). Finally, a lipid bilayer was coated on the surface of the LAB-gel to improve the stability of the microgel in gastric juice. Engineered probiotics survived an oxidative stress insult (100 μM H2O2) and were able to effectively colonize the colon. Furthermore, engineered probiotics could significantly uptake by macrophages and scavenge intracellular ROS. Engineered probiotics with enhanced colonization and ROS scavenging activity showed promising therapeutic effects on colitis.

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工程益生菌通过改善肠道定植和清除ROS治疗结肠炎。

益生菌在治疗结肠炎方面具有重要的前景，但由于其在结肠直肠氧化应激中的定植和存活不足，其应用仍然具有挑战性。本研究制备了工程益生菌，以增强益生菌的定植，清除结肠炎部位过表达的活性氧（ROS）。首先，鼠李糖乳杆菌（Lactobacillus rhamnosus， LAB）表面的初级氨基通过简单的一步胺酯反应转化为游离硫醇。然后，我们利用巯基引发的二硫代烷开环级联，通过α -硫辛酸（LA）和硫辛酸钠（LANa）的反应，在LAB表面制备了一类结构动态微凝胶（LAB-gel）。最后，在lab -凝胶表面包被一层脂质双分子层，以提高微凝胶在胃液中的稳定性。工程益生菌在氧化应激损伤（100 μM H2O2）中存活，并能够有效地定植结肠。此外，工程益生菌可显著被巨噬细胞摄取，清除细胞内活性氧。具有增强定植和清除活性氧活性的工程益生菌对结肠炎有良好的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Macro Letters 化学-

CiteScore

10.40

自引率

3.40%

发文量

209

审稿时长

1 months

期刊介绍： ACS Macro Letters publishes research in all areas of contemporary soft matter science in which macromolecules play a key role, including nanotechnology, self-assembly, supramolecular chemistry, biomaterials, energy generation and storage, and renewable/sustainable materials. Submissions to ACS Macro Letters should justify clearly the rapid disclosure of the key elements of the study. The scope of the journal includes high-impact research of broad interest in all areas of polymer science and engineering, including cross-disciplinary research that interfaces with polymer science. With the launch of ACS Macro Letters, all Communications that were formerly published in Macromolecules and Biomacromolecules will be published as Letters in ACS Macro Letters.