Multi-locus molecular phylogenetic analysis reveals four new species and a new record of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) on dipteran hosts in Thailand.

IF 3.6 2区生物学 Q2 MYCOLOGY

Mycokeys Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI:10.3897/mycokeys.119.155439

Suchada Mongkolsamrit, Donnaya Thanakitpipattana, Wasana Noisripoom, Kanoksri Tasanathai, Kanraya Liangsiri, Somruetai Jaiyen, Nattawut Rungjindamai, Marc Stadler, Jennifer Luangsa-Ard

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Abstract

During field surveys conducted in various regions of Thailand, several fungal specimens parasitising flies were discovered. These fungi exhibited morphological characteristics consistent with the broad concept of Ophiocordycepsdipterigena, including yellow to orange-brown cylindrical stromata bearing fertile ascomata at the tip. Multi-locus phylogenetic analyses based on ITS, LSU, TEF1, and RPB2 sequences revealed that these specimens belong to a well-supported monophyletic clade, referred to as the 'O.dipterigena' complex, which forms a distinct subclade within the hymenostilboid clade. This complex comprises four newly described species with clearly defined host associations: O.floriformis, found on robber flies (Asilidae), particularly on Clephydroneura sp.; O.muscae, isolated from the housefly (Muscadomestica); O.tabani, parasitising horse flies (Tabanus sp.); and O.thilosuensis, infecting fruit flies (Anastrephaobliqua) and soldier flies (Sarginae). Additionally, several strains clustered with the previously described O.philippinensis, which was also collected from Clephydroneura sp. This marks the first record of O.philippinensis in Thailand.

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Multi-locus分子系统发育分析揭示了在泰国双翅目寄主上的4个新种和1个新记录。

在泰国不同地区进行的实地调查中，发现了几种寄生于苍蝇的真菌标本。这些真菌的形态特征与Ophiocordycepsdipterigena的广义概念一致，包括黄色到橙棕色的圆柱形基质，顶端有可育的ascomata。基于ITS、LSU、TEF1和RPB2序列的多位点系统发育分析显示，这些标本属于一个支持良好的单系分支，被称为“O.dipterigena”复合体，它在膜静肢类分支中形成了一个独特的亚分支。该复合体包括四种新描述的物种，它们与宿主有明确的联系：O.floriformis，发现于强盗蝇（Asilidae），特别是在Clephydroneura sp.；muscae，从家蝇（muscadmestica）中分离；寄生于马蝇的tabani (Tabanus sp.)；和othilosuensis，感染果蝇（Anastrephaobliqua）和兵蝇（Sarginae）。此外，有几株菌株与先前报道的菲律宾蠓属聚集在一起，这也是在Clephydroneura sp.中收集到的菲律宾蠓属。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mycokeys MYCOLOGY-

CiteScore

5.90

自引率

12.10%

发文量

审稿时长

6 weeks

期刊介绍： MycoKeys is a peer-reviewed, open-access, online and print, rapidly produced journal launched to support free exchange of ideas and information in systematics and biology of fungi (including lichens). All papers published in MycoKeys can be freely copied, downloaded, printed and distributed at no charge for the reader. Authors are thus encouraged to post the pdf files of published papers on their homepages or elsewhere to expedite distribution. There are no restrictions nor charge for color.

﻿Multi-locus molecular phylogenetic analysis reveals four new species and a new record of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) on dipteran hosts in Thailand.

Abstract

Multi-locus molecular phylogenetic analysis reveals four new species and a new record of Ophiocordyceps (Ophiocordycipitaceae, Hypocreales) on dipteran hosts in Thailand.