In vitro study on the osteoimmunological potential of magnesium implants (WE43MEO).

IF 2.9 4区 医学 Q3 ENGINEERING, BIOMEDICAL
Sabrin Aydin, Ana Prates Soares, Heilwig Fischer, Raphael Silvan Knecht, Alexander Kopp, Katharina Schmidt-Bleek, Max Heiland, Carsten Rendenbach
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引用次数: 0

Abstract

Introduction: Bioresorbable implants significantly advance orthopedics and regenerative medicine, offering advantages over permanent implants for bone regeneration. They eliminate the need for secondary surgery and reduce long-term risks associated with permanent implants. Magnesium-based alloys are particularly promising, as their biocompatibility and mechanical properties are similar to bone. However, the degradation of magnesium is associated with physiological challenges that need to be better understood.

Objective: The primary focus of this in vitro study was to investigate the osteogenic and immunomodulatory potential of WE43, a promising magnesium alloy tailored for clinical applications, and to test its osteogenic effect when a plasma electrolytic oxidation (PEO) surface modification is added.

Results: The present data revealed that WE43 implants show excellent biocompatibility and bioactivity, promoting the viability of osteoblasts and enhancing the expression of osteogenic genes, specially Alpl and Tnfrsf11b. PEO surface modification did not further enhance osteogenic differentiation. Notably, WE43 implants elicited a minimal inflammatory response in RAW264.7 murine macrophages, indicating good biocompatibility. Furthermore, supernatant collected from RAW264.7 murine macrophages cultured with WE43 implants stimulated the Alpl expression in MC3T3-E1 murine osteoblasts, demonstrating their potential osteoimmune effect.

Conclusion: The present findings highlight the promising potential of WE43 alloy as a biocompatible and osteoinductive biomaterial for bone regeneration applications. Their osteoimmune modulation further demonstrates the advantages of using this alloy system. Specifically, a minimal, well-controlled inflammatory response can promote a faster transition to the bone remodeling phase, leading to quicker and more effective bone regeneration.

Methodology: A comprehensive in vitro investigation was conducted to assess the impact of both WE43 and WE43 PEO on the viability, Alkaline Phosphatase (ALP) expression, osteogenic gene expression (Alpl, Tnfrsf11b, and Bglap), and mineralization of MC3T3-E1 murine osteoblasts. The osteoimmunomodulatory response to WE43 was evaluated using RAW264.7 murine macrophages by assessing their response to direct contact with the alloy.

镁植入物(WE43MEO)骨免疫潜能的体外研究。
生物可吸收植入物显著推进骨科和再生医学,提供比永久植入物更有利的骨再生。它们消除了二次手术的需要,并降低了与永久植入物相关的长期风险。镁基合金尤其有前景,因为它们的生物相容性和机械性能与骨相似。然而,镁的降解与生理挑战有关,需要更好地理解。目的:研究临床应用前景广阔的镁合金WE43的成骨和免疫调节潜能,并检测其在加入等离子体电解氧化(PEO)表面修饰后的成骨效果。结果:WE43植入物具有良好的生物相容性和生物活性,促进了成骨细胞的活力,增强了成骨基因,特别是Alpl和Tnfrsf11b的表达。PEO表面修饰不能进一步促进成骨分化。值得注意的是,WE43植入物在RAW264.7小鼠巨噬细胞中引起的炎症反应最小,表明其具有良好的生物相容性。此外,WE43植入物培养的RAW264.7小鼠巨噬细胞上清可刺激MC3T3-E1小鼠成骨细胞中Alpl的表达,显示其潜在的骨免疫作用。结论:WE43合金是一种具有生物相容性和骨诱导功能的骨再生材料,具有广阔的应用前景。它们的骨免疫调节进一步证明了使用该合金系统的优势。具体来说,一个最小的、控制良好的炎症反应可以促进更快地过渡到骨重塑阶段,从而更快、更有效地实现骨再生。方法:通过全面的体外研究,评估WE43和WE43 PEO对MC3T3-E1小鼠成骨细胞活力、碱性磷酸酶(ALP)表达、成骨基因(Alpl、Tnfrsf11b和Bglap)表达和矿化的影响。采用RAW264.7小鼠巨噬细胞,通过评估其与合金直接接触的反应来评估其对WE43的骨免疫调节反应。
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来源期刊
BioMedical Engineering OnLine
BioMedical Engineering OnLine 工程技术-工程:生物医学
CiteScore
6.70
自引率
2.60%
发文量
79
审稿时长
1 months
期刊介绍: BioMedical Engineering OnLine is an open access, peer-reviewed journal that is dedicated to publishing research in all areas of biomedical engineering. BioMedical Engineering OnLine is aimed at readers and authors throughout the world, with an interest in using tools of the physical and data sciences and techniques in engineering to understand and solve problems in the biological and medical sciences. Topical areas include, but are not limited to: Bioinformatics- Bioinstrumentation- Biomechanics- Biomedical Devices & Instrumentation- Biomedical Signal Processing- Healthcare Information Systems- Human Dynamics- Neural Engineering- Rehabilitation Engineering- Biomaterials- Biomedical Imaging & Image Processing- BioMEMS and On-Chip Devices- Bio-Micro/Nano Technologies- Biomolecular Engineering- Biosensors- Cardiovascular Systems Engineering- Cellular Engineering- Clinical Engineering- Computational Biology- Drug Delivery Technologies- Modeling Methodologies- Nanomaterials and Nanotechnology in Biomedicine- Respiratory Systems Engineering- Robotics in Medicine- Systems and Synthetic Biology- Systems Biology- Telemedicine/Smartphone Applications in Medicine- Therapeutic Systems, Devices and Technologies- Tissue Engineering
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