Metal chelation approach synergized with surfactant for spectrofluorimetric assay of moxifloxacin with method greenness evaluation.

Abstract

This work deals with devising a quick and easy method for moxifloxacin assay using a sensitive spectrofluorimetric technique. The method benefited from the interaction of the aforementioned drug with the aluminum cation which form a stable chelate with a high fluorescence characteristic. Further improvement in the fluorescence was achieved upon the addition of sodium lauryl sulfate. The experimental parameters of the reaction were carefully investigated. The fluorescence intensity was measured at a wavelength of 478 nm after excitation at 365 nm. A linear relationship between the intensity of fluorescence and moxifloxacin concentration was in the range of 10-200 ng mL^- 1. The developed method was highly sensitive since the lower limit of detection was 2.2 ng mL^- 1. Application of the suggested spectrofluorimetric method included the analysis of commercial tablet dosage forms containing the cited drug. In addition, human plasma samples spiked with the drug were analyzed, and good recovery was obtained. Moreover, the environmental friendliness of the developed procedure was confirmed through employing AGREE and GAPI approaches.