Fate and enzymatic response of co-exposed photoaged nanoplastic and PFAS: Insights from a human gastrointestinal simulation

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-07-16 DOI:10.1016/j.jhazmat.2025.139274

Qian Yang , Lanqing Xu , Xiaofan Yang , Yinglin Liu , Junyi Zhang , Mengna Tao , Tao Chen , Xuesong Cao , Zhenyu Wang

{"title":"Fate and enzymatic response of co-exposed photoaged nanoplastic and PFAS: Insights from a human gastrointestinal simulation","authors":"Qian Yang , Lanqing Xu , Xiaofan Yang , Yinglin Liu , Junyi Zhang , Mengna Tao , Tao Chen , Xuesong Cao , Zhenyu Wang","doi":"10.1016/j.jhazmat.2025.139274","DOIUrl":null,"url":null,"abstract":"<div><div>This study investigated the bioaccessibility and enzymatic response of nanoplastics (NPs) and per- and poly-fluoroalkyl substances (PFAS) with different chain length and functional groups during co-exposure in simulated human gastrointestinal digestion. The important role of phototransformation of NPs in these processes was also assessed. Results showed that the bioaccessibility of photoaged NPs (ANPs) in the oral, gastric, and intestinal phases was 51.58-, 1.39-, and 5.50-fold of NPs, respectively. Co-exposure of ANPs and short-chain PFBA significantly increased the bioaccessibility of ANPs in the oral and the intestinal phases by 56.41 % and 41.98 %, respectively, and the bioaccessibility of PFBA was increased by 27.58 % and 163.57 %. However, in the gastric phase, co-exposure significantly reduced the bioavailability of PFAS, NPs, and ANPs by 10.77 %-93.61 %, 9.21 %-42.38 %, and 55.07 %-83.00 %, respectively. Pepsin activity was also significantly inhibited by 11.40 %–16.32 % in co-exposure treatments. Atomic force microscopy-infrared spectroscopy revealed that ANPs underwent surface chemical modifications in the gastric phase, with subsequent altering their interaction with pepsin. Molecular docking results showed that NPs primarily binds to pepsin through hydrophobic interaction near the tyrosine and alanine residues, and NPs+PFOA showed the highest binding stability with pepsin. The findings enhance the understanding of human health of co-exposed NPs and PFAS.</div></div>","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":"496 ","pages":"Article 139274"},"PeriodicalIF":11.3000,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hazardous Materials","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304389425021909","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

This study investigated the bioaccessibility and enzymatic response of nanoplastics (NPs) and per- and poly-fluoroalkyl substances (PFAS) with different chain length and functional groups during co-exposure in simulated human gastrointestinal digestion. The important role of phototransformation of NPs in these processes was also assessed. Results showed that the bioaccessibility of photoaged NPs (ANPs) in the oral, gastric, and intestinal phases was 51.58-, 1.39-, and 5.50-fold of NPs, respectively. Co-exposure of ANPs and short-chain PFBA significantly increased the bioaccessibility of ANPs in the oral and the intestinal phases by 56.41 % and 41.98 %, respectively, and the bioaccessibility of PFBA was increased by 27.58 % and 163.57 %. However, in the gastric phase, co-exposure significantly reduced the bioavailability of PFAS, NPs, and ANPs by 10.77 %-93.61 %, 9.21 %-42.38 %, and 55.07 %-83.00 %, respectively. Pepsin activity was also significantly inhibited by 11.40 %–16.32 % in co-exposure treatments. Atomic force microscopy-infrared spectroscopy revealed that ANPs underwent surface chemical modifications in the gastric phase, with subsequent altering their interaction with pepsin. Molecular docking results showed that NPs primarily binds to pepsin through hydrophobic interaction near the tyrosine and alanine residues, and NPs+PFOA showed the highest binding stability with pepsin. The findings enhance the understanding of human health of co-exposed NPs and PFAS.

Abstract Image

查看原文本刊更多论文

光老化纳米塑料和PFAS共暴露的命运和酶的反应：从人类胃肠模拟的见解

本研究研究了纳米塑料（NPs）与不同链长和官能团的单氟烷基和多氟烷基物质（PFAS）在模拟人体胃肠消化过程中共暴露的生物可及性和酶促反应。并对NPs光转化在这些过程中的重要作用进行了评价。结果表明，光老化NPs （ANPs）在口腔、胃和肠期的生物可及性分别为NPs的51.58倍、1.39倍和5.50倍。ANPs与短链PFBA共暴露可显著提高ANPs在口腔期和肠道期的生物可及性，分别提高56.41%和41.98%，PFBA的生物可及性分别提高27.58%和163.57%。然而，在胃期，共暴露显著降低了PFAS、NPs和ANPs的生物利用度，分别为10.77% ~ 93.61%、9.21% ~ 42.38%和55.07% ~ 83.00%。共暴露处理对胃蛋白酶活性也有11.40% ~ 16.32%的显著抑制作用。原子力显微镜-红外光谱显示，ANPs在胃期发生了表面化学修饰，随后改变了它们与胃蛋白酶的相互作用。分子对接结果表明，NPs主要通过酪氨酸和丙氨酸残基附近的疏水相互作用与胃蛋白酶结合，NPs+PFOA与胃蛋白酶的结合稳定性最高。研究结果加强了对共暴露的NPs和PFAS的人类健康的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.