[Genomic characterization of a case of enterovirus D68 infection in a child from Tongzhou District, Beijing City].

Q3 Medicine

中华预防医学杂志 Pub Date : 2025-07-06 DOI:10.3760/cma.j.cn112150-20250326-00247

B J Zhen, P Zhang, X C Guo, J Zhang, Y Zhang, X Gao, F Wang, J Li, L Zou

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Abstract

A throat swab sample from a pediatric case in Tongzhou District, Beijing was identified as enterovirus; the patient was a 1-year-and-8-month-old male sporadic case. Whole-genome sequencing revealed a viral genome length of 7 436 bp. BLAST alignment confirmed the serotype as EV-D68. Phylogenetic analysis of the whole genome indicated that this strain belongs to the B3 clade, showing closer genetic proximity to the 2018 Shanghai strain MW697453 with 99.53% whole-genome nucleotide homology. Genetic and amino acid variation analysis demonstrated that the B3 subclade to which this strain belongs exhibits a nucleotide deletion at positions 718-726, differing from deletion sites observed in other B3 clade strains. A key neuropathogenic amino acid site, T650A, was found to have undergone mutation. Recombination analysis confirmed no cross-clade recombination events in this strain. This study conducted genetic characterization of the strain's evolutionary relationship with EV-D68 strains from different regions and years in China, providing data support for formulating prevention and control measures against EV-D68 infection.

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[北京市通州区1例儿童肠道病毒D68感染的基因组特征分析]。

北京市通州区1例小儿病例咽拭子样本被鉴定为肠道病毒；患者为1岁零8个月男性散发病例。全基因组测序显示该病毒基因组长度为7 436 bp。BLAST比对证实血清型为EV-D68。全基因组系统发育分析表明，该菌株属于B3支系，与2018年上海菌株MW697453遗传接近度更高，全基因组同源性为99.53%。遗传和氨基酸变异分析表明，该菌株所属的B3亚枝在718-726处存在核苷酸缺失，与其他B3亚枝菌株的缺失位点不同。一个关键的神经致病氨基酸位点T650A被发现发生了突变。重组分析证实该菌株无跨枝重组事件。本研究对该菌株与中国不同地区、不同年份EV-D68菌株的进化关系进行遗传鉴定，为制定EV-D68感染防控措施提供数据支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中华预防医学杂志 Medicine-Medicine (all)

CiteScore

1.20

自引率

0.00%

发文量

12678

期刊介绍： Chinese Journal of Preventive Medicine (CJPM), the successor to Chinese Health Journal , was initiated on October 1, 1953. In 1960, it was amalgamated with the Chinese Medical Journal and the Journal of Medical History and Health Care , and thereafter, was renamed as People’s Care . On November 25, 1978, the publication was denominated as Chinese Journal of Preventive Medicine . The contents of CJPM deal with a wide range of disciplines and technologies including epidemiology, environmental health, nutrition and food hygiene, occupational health, hygiene for children and adolescents, radiological health, toxicology, biostatistics, social medicine, pathogenic and epidemiological research in malignant tumor, surveillance and immunization.