Harnessing CRISPR/Cas9 in engineering biotic stress immunity in crops.

IF 3.8 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2025-07-15 DOI:10.1007/s00425-025-04769-z
Bayram Ali Yerlikaya, Seher Yerlikaya, Batuhan Gül, Hümeyra Yoldaş, Musa Kavas, Heba I Mohamed
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引用次数: 0

Abstract

Main conclusion: There is significant potential for CRISPR/Cas9 to be used in developing crops that can adapt to biotic stresses such as fungal, bacterial, viral, and pest infections and weeds. The increasing global population and climate change present significant threats to food security by putting stress on plants, making them more vulnerable to diseases and productivity losses caused by pathogens, pests, and weeds. Traditional breeding methods are inadequate for the rapid development of new plant traits needed to counteract this decline in productivity. However, modern advances in genome-editing technologies, particularly CRISPR/Cas9, have transformed crop protection through precise and targeted modifications of plant genomes. This enables the creation of resilient crops with improved resistance to pathogens, pests, and weeds. This review examines various methods by which CRISPR/Cas9 can be utilized for crop protection. These methods include knocking out susceptibility genes, introducing resistance genes, and modulating defense genes. Potential applications of CRISPR/Cas9 in crop protection involve introducing genes that confer resistance to pathogens, disrupting insect genes responsible for survival and reproduction, and engineering crops that are resistant to herbicides. In conclusion, CRISPR/Cas9 holds great promise for advancing crop protection and ensuring food security in the face of environmental challenges and increasing population pressures. The most recent advancements in CRISPR technology for creating resistance to bacteria, fungi, viruses, and pests are covered here. We wrap up by outlining the most pressing issues and technological shortcomings, as well as unanswered questions for further study.

利用CRISPR/Cas9在作物生物胁迫免疫工程中的应用
主要结论:CRISPR/Cas9在开发能够适应真菌、细菌、病毒、害虫感染和杂草等生物胁迫的作物方面具有巨大的潜力。全球人口增长和气候变化给植物带来压力,使它们更容易受到病原体、害虫和杂草造成的疾病和生产力损失的影响,从而对粮食安全构成重大威胁。传统的育种方法不足以快速发展新的植物性状来抵消这种生产力的下降。然而,基因组编辑技术的现代进步,特别是CRISPR/Cas9,通过对植物基因组进行精确和有针对性的修改,已经改变了作物保护。这样就可以培育出具有抗病性的作物,提高对病原体、害虫和杂草的抵抗力。本文综述了CRISPR/Cas9用于作物保护的各种方法。这些方法包括敲除易感基因、引入抗性基因和调节防御基因。CRISPR/Cas9在作物保护中的潜在应用包括引入对病原体具有抗性的基因,破坏负责生存和繁殖的昆虫基因,以及设计对除草剂具有抗性的作物。总之,在面临环境挑战和日益增长的人口压力的情况下,CRISPR/Cas9在推进作物保护和确保粮食安全方面具有巨大的前景。这里介绍了CRISPR技术在抵抗细菌、真菌、病毒和害虫方面的最新进展。最后,我们概述了最紧迫的问题和技术缺陷,以及有待进一步研究的未解决问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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