Genome-wide identification of the C2-domain abscisic acid-related (CAR) protein family in Rosaceae and functional characterization of PbrCAR1 in pear pollen tube growth.

IF 3.8 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2025-07-15 DOI:10.1007/s00425-025-04764-4
Xueying Liu, Yanhong Zhang, Hong Su, Baofeng Zhang, Fengjun Zhao, Hao Zhang, Chao Tang, Peng Wang, Shaoling Zhang, Wenjie Zhou, Juyou Wu
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引用次数: 0

Abstract

Main conclusion: This study identified a PbrCAR1 gene in pear, which promotes pollen tube growth by mediating vesicle transport, and Ca2+ treatment enhances PbrCAR1 expression and promotes its plasma membrane accumulation. The C2-domain abscisic acid-related (CAR) proteins bind to diverse signaling protein complexes and play essential roles in numerous biological processes, including blue light tropism, gravitropism, iron nutrition, and responses to biotic and abiotic stresses. However, the functions of CAR gene family members in pear pollen development within the Rosaceae family remain inadequately explored. In this study, we identified a total of 33 CAR genes across four Rosaceae species. Phylogenetic and structural analyses classified these genes into three distinct subfamilies (I to III). Evolutionary analyses indicated that purifying selection significantly influenced the evolution of the Rosaceae CAR gene family, while whole-genome duplication and tandem duplication contributed substantially to its expansion. Transcriptomic data combined with quantitative real-time PCR (qRT-PCR) analysis revealed tissue-specific expression patterns of PbrCAR genes, with PbrCAR1 showing high expression during pollen development and mainly localizing in the cytoplasm. Exogenous calcium ion (Ca2⁺) treatment was shown to enhance the expression of PbrCAR1 and promote its accumulation at the plasma membrane. Moreover, knocking down PbrCAR1 in pear pollen tubes significantly inhibited vesicle trafficking at the pollen tube tip, thereby suppressing pollen tube growth. These findings indicate that PbrCAR1 regulates pear pollen tube growth by mediating vesicle transport and provide valuable insights along with a theoretical foundation for studying the evolution, expression patterns, and functions of the CAR gene family in plants.

蔷薇科c2结构域脱落酸相关蛋白家族的全基因组鉴定及PbrCAR1在梨花粉管生长中的功能表征
主要结论:本研究在梨中鉴定出PbrCAR1基因,该基因通过介导囊泡运输促进花粉管生长,Ca2+处理可增强PbrCAR1的表达,促进其质膜积累。c2结构域脱落酸相关(CAR)蛋白与多种信号蛋白复合物结合,并在许多生物过程中发挥重要作用,包括蓝光趋向性、向地性、铁营养以及对生物和非生物胁迫的反应。然而,CAR基因家族成员在玫瑰科梨花粉发育中的作用尚未得到充分的研究。在这项研究中,我们在四个蔷薇科物种中共鉴定了33个CAR基因。系统发育和结构分析将这些基因分为三个不同的亚家族(I到III)。进化分析表明,纯化选择显著影响了蔷薇科CAR基因家族的进化,而全基因组重复和串联重复对其扩增有重要作用。转录组学数据结合实时荧光定量PCR (qRT-PCR)分析揭示了PbrCAR基因的组织特异性表达模式,PbrCAR1在花粉发育过程中高表达,主要定位于细胞质中。外源性钙离子(Ca2 +)处理可以增强PbrCAR1的表达,并促进其在质膜处的积累。此外,敲低梨花粉管中的PbrCAR1可显著抑制花粉管尖端的囊泡运输,从而抑制花粉管的生长。这些发现表明PbrCAR1通过介导囊泡运输调控梨花粉管的生长,为研究植物中CAR基因家族的进化、表达模式和功能提供了有价值的理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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