Clinical significance of lncRNA PAX8-AS1 and miR-96-5p in non-small cell lung cancer.

IF 1.5 4区医学 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS

Journal of Cardiothoracic Surgery Pub Date : 2025-07-14 DOI:10.1186/s13019-025-03542-3

Qiaoling Ying, Hui Xu, Xiaojiao Wu, Hang Fang, Jingjing Shi, Hangcheng Pan

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引用次数: 0

Abstract

Background: This study aims to examine the potential value of lncRNA PAX8-AS1 and miR-96-5p as diagnostic markers in non-small cell lung cancer (NSCLC). The goal is to provide a reference for improving adverse outcomes in NSCLC and developing effective early interventions in the clinic.

Methods: A total of 112 NSCLC patients and 70 healthy volunteers were recruited as the study subjects. The expression levels of PAX8-AS1 and miR-96-5p were measured using fluorescence quantitative PCR. The expression levels of PAX8-AS1 and miR-96-5p were detected by fluorescence quantitative PCR. A dual luciferase reporter assay was used to detect the binding of PAX8-AS1 and miR-96-5p. ROC, Kaplan-Meier, and Cox regression analysis was used to assess the diagnostic and prognostic value of PAX8-AS1 and miR-96-5p in NSCLC.

Results: In NSCLC, PAX8-AS1 was found to be downregulated, whereas miR-96-5p was upregulated. These two molecules exhibited a negative correlation and a target-binding relationship. Receiver operating characteristic (ROC) curve analysis demonstrated that PAX8-AS1 and miR-96-5p had practical diagnostic values for distinguishing NSCLC patients. Mortality increased in NSCLC patients with low PAX8-AS1 levels and decreased in those with low miR-96-5p levels. Cox regression analysis showed that PAX8-AS1 and miR-96-5p could serve as independent predictors of prognostic survival in NSCLC patients.

Conclusion: PAX8-AS1 and miR-96-5p can serve as potential diagnostic biomarkers for NSCLC patients.

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lncRNA PAX8-AS1和miR-96-5p在非小细胞肺癌中的临床意义

背景：本研究旨在探讨lncRNA PAX8-AS1和miR-96-5p作为非小细胞肺癌（NSCLC）诊断标志物的潜在价值。目的是为改善非小细胞肺癌的不良结局和制定有效的临床早期干预措施提供参考。方法：选取112例非小细胞肺癌患者和70名健康志愿者作为研究对象。采用荧光定量PCR检测PAX8-AS1和miR-96-5p的表达水平。采用荧光定量PCR检测PAX8-AS1和miR-96-5p的表达水平。采用双荧光素酶报告试验检测PAX8-AS1与miR-96-5p的结合。采用ROC、Kaplan-Meier和Cox回归分析评估PAX8-AS1和miR-96-5p在NSCLC中的诊断和预后价值。结果：在NSCLC中，PAX8-AS1下调，而miR-96-5p上调。这两个分子表现出负相关和靶结合关系。受试者工作特征（ROC）曲线分析显示PAX8-AS1和miR-96-5p对区分NSCLC患者具有实用的诊断价值。低PAX8-AS1水平的NSCLC患者死亡率升高，低miR-96-5p水平的NSCLC患者死亡率降低。Cox回归分析显示PAX8-AS1和miR-96-5p可作为NSCLC患者预后生存的独立预测因子。结论：PAX8-AS1和miR-96-5p可作为非小细胞肺癌患者的潜在诊断生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Cardiothoracic Surgery 医学-心血管系统

CiteScore

2.50

自引率

6.20%

发文量

286

审稿时长

4-8 weeks

期刊介绍： Journal of Cardiothoracic Surgery is an open access journal that encompasses all aspects of research in the field of Cardiology, and Cardiothoracic and Vascular Surgery. The journal publishes original scientific research documenting clinical and experimental advances in cardiac, vascular and thoracic surgery, and related fields. Topics of interest include surgical techniques, survival rates, surgical complications and their outcomes; along with basic sciences, pediatric conditions, transplantations and clinical trials. Journal of Cardiothoracic Surgery is of interest to cardiothoracic and vascular surgeons, cardiothoracic anaesthesiologists, cardiologists, chest physicians, and allied health professionals.