{"title":"The Clinical Value of hsa_circ_0000419 in Diagnosis and Prognosis of Esophageal Squamous Cell Carcinoma.","authors":"Yuanmei Dong, Huiling Meng, Yijia Chen, Huiwen Guo, Xiaoming Shi, Qibin He, Dongliang Mao","doi":"10.1007/s10528-025-11194-0","DOIUrl":null,"url":null,"abstract":"<p><p>The 5-year survival rates of esophageal squamous carcinoma (ESCC) patients were pessimistic. Hsa_circ_0000419 could influence the malignant behavior of many cancers. To evaluate whether hsa_circ_0000419 can be an independent indicator for ESCC diagnosis and prognosis. Using RT-qPCR to determine hsa_circ_0000419 and miR-580-3p expression in ESCC tissues and cells. The role of hsa_circ_0000419 for ESCC patients' diagnosis and prognosis was assessed by ROC curve, Kaplan-Meier curve, and Cox regression analysis. The functions of hsa_circ_0000419 in KYSE450 and TE13 cells were tested by the CCK-8 method, transwell method, and flow cytometry. The relationship between hsa_circ_0000419 and miR-580-3p was verified by dual-luciferase reporter assay. Our study revealed that the level of hsa_circ_0000419 was elevated in ESCC tissues and cell lines. hsa_circ_0000419 ROC curves could effectively distinguish between tumor tissues and normal tissues. Kaplan-Meier curve indicated that ESCC patients had a poor prognosis with high-hsa_circ_0000419 level. The Cox regression analysis showed that hsa_circ_0000419 was an independent biomarker for ESCC. In vitro assay revealed that inhibiting hsa_circ_0000419 expression controlled the malignant behavior of ESCC, while miR-580-3p inhibitor could reverse this inhibition. hsa_circ_0000419 promoted malignant behavior in ESCC by sponging miR-580-3p. Hsa_circ_0000419 was an indicator for the diagnosis and prognosis of ESCC. Hsa_circ_0000419 promoted ESCC cell proliferation, migration, and invasion, and inhibited apoptosis by down-regulating miR-580-3p expression.</p>","PeriodicalId":482,"journal":{"name":"Biochemical Genetics","volume":" ","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10528-025-11194-0","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The 5-year survival rates of esophageal squamous carcinoma (ESCC) patients were pessimistic. Hsa_circ_0000419 could influence the malignant behavior of many cancers. To evaluate whether hsa_circ_0000419 can be an independent indicator for ESCC diagnosis and prognosis. Using RT-qPCR to determine hsa_circ_0000419 and miR-580-3p expression in ESCC tissues and cells. The role of hsa_circ_0000419 for ESCC patients' diagnosis and prognosis was assessed by ROC curve, Kaplan-Meier curve, and Cox regression analysis. The functions of hsa_circ_0000419 in KYSE450 and TE13 cells were tested by the CCK-8 method, transwell method, and flow cytometry. The relationship between hsa_circ_0000419 and miR-580-3p was verified by dual-luciferase reporter assay. Our study revealed that the level of hsa_circ_0000419 was elevated in ESCC tissues and cell lines. hsa_circ_0000419 ROC curves could effectively distinguish between tumor tissues and normal tissues. Kaplan-Meier curve indicated that ESCC patients had a poor prognosis with high-hsa_circ_0000419 level. The Cox regression analysis showed that hsa_circ_0000419 was an independent biomarker for ESCC. In vitro assay revealed that inhibiting hsa_circ_0000419 expression controlled the malignant behavior of ESCC, while miR-580-3p inhibitor could reverse this inhibition. hsa_circ_0000419 promoted malignant behavior in ESCC by sponging miR-580-3p. Hsa_circ_0000419 was an indicator for the diagnosis and prognosis of ESCC. Hsa_circ_0000419 promoted ESCC cell proliferation, migration, and invasion, and inhibited apoptosis by down-regulating miR-580-3p expression.
期刊介绍：
Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses.
Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication.
Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses.
Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods.
Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.
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