Production of Polymeric Membranes Functionalized with Vitreous Humor and Secretome from Dental Pulp Stem Cells for Use in Epithelial Regeneration.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-07-15 DOI:10.1021/acsabm.5c00743

Wallady da Silva Barroso, Erika Patrícia Chagas Gomes Luz, Lidyane Souto Maciel Marques, Paulo Eduardo da Silva Cavalcante, Francisco Fábio Pereira de Souza, Adriano Lincoln Albuquerque Mattos, Fabia Karine Andrade, André Luís Coelho da Silva, Mariáh Cationi Hirata, Isadora Bosco, Daniel Navarro da Rocha, Renata Francielle Bombaldi de Souza, Fernanda Carla Bombaldi de Souza, José Ricardo Muniz Ferreira, Rodrigo Silveira Vieira

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引用次数: 0

Abstract

This study investigated the development of porous membranes based on a matrix of bacterial cellulose (BC) and sodium alginate (ALG) incorporated with proteins extracted from the tuna vitreous humor and complete conditioned medium (CCM) obtained from the in vitro culture of dental pulp stem cells. These bioactive components were integrated into BC-ALG membranes, which were then evaluated for morphology, physicochemical properties, in vitro cytotoxicity, and regenerative potential in vitro. The membranes exhibited high porosity (72% to 89%), and remarkable swelling capacity reaching up to 3376 ± 0.78% in 72 min, indicating excellent moisture retention essential for wound healing in vivo. Cytotoxicity assay showed that none of the formulations were cytotoxic to the tested mammalian cells, confirming their cytocompatibility. Membranes containing CCM showed enhanced cell viability at lower concentrations (500 μg/mL and 250 μg/mL). In vitro scratch assays demonstrated that membranes with vitreous humor (VH) and CCM reduced scratch closure time from 18 to 12 h, suggesting their potential for accelerating skin regeneration. This work underscores the promise of using sustainable, waste-derived materials for creating bioactive membranes with applications in tissue engineering and regenerative medicine.

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用牙髓干细胞制备玻璃体和分泌组功能化聚合膜用于上皮细胞再生。

本研究以细菌纤维素（BC）和海藻酸钠（ALG）为基质，结合从金枪鱼玻璃体中提取的蛋白质和牙髓干细胞体外培养的完全条件培养基（CCM）为基质，研究了多孔膜的形成。这些生物活性成分被整合到BC-ALG膜中，然后对其形态、理化性质、体外细胞毒性和体外再生潜力进行评估。膜具有较高的孔隙率（72% ~ 89%），在72 min内溶胀率可达3376±0.78%，表明膜具有良好的保湿性，是伤口在体内愈合所必需的。细胞毒性试验表明，这些制剂对实验动物细胞均无细胞毒性，证实了它们的细胞相容性。含CCM的膜在较低浓度（500 μg/mL和250 μg/mL）下细胞活力增强。体外划痕实验表明，含有玻璃体间质（VH）和CCM的膜将划痕闭合时间从18小时缩短到12小时，这表明它们具有加速皮肤再生的潜力。这项工作强调了使用可持续的废物衍生材料来制造生物活性膜在组织工程和再生医学中的应用前景。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.