OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience

IF 1.9 Q3 INFECTIOUS DISEASES

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Pub Date : 2025-07-14 DOI:10.1016/j.jctube.2025.100552

Senjuti Kabir , Sabrina Choudhury , Mohammad Khaja Mafij Uddin , M.D. Fahim Ather , Syed Mohammad Mazidur Rahman , Pronab Kumar Modak , Mohammad Samsuddoha Sarker Shanchay , Tanjina Rahman , Shahriar Ahmed , Sayera Banu

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引用次数: 0

Abstract

Background

Performance of OMNIgene.SPUTUM (OM-S) for transporting sputum was evaluated.

Methods

This exploratory study was conducted during January-December 2019 at four near and one distant healthcare-facilities of Dhaka. Smear-positive pulmonary TB patients’ sputa were collected, divided into ‘OM-S untreated’ and ‘OM-S treated’ portions, and transported to testing laboratory, Dhaka, on same-day from near-sites, and through courier from distant-site for smear-microscopy, culture, and Xpert MTB/RIF (Xpert) testing. Subset of ‘OM-S treated’ sample was tested with Xpert without centrifugation. Test results of all portions were compared in between.

Results

Total 444 participants were enrolled (near-sites:198, distant-site: 246). All test results were comparable in both portions for near-sites. For distant-site, smear-microscopy’s positivity was reduced by 4.1 % in ‘OM-S treated’, Xpert showed 100 % concordance in both portions, and culture was higher in ‘OM-S treated’ than ‘OM-S untreated’ (92.3 % vs 89.4 %; p = 0.288). Primary contamination rate in ‘OM-S treated’ was lower than ‘OM-S untreated’ (2.0 % vs 9.8 %; p < 0.05). For all sites, median (IQR) time-to-culture positivity was 35 (28, 42) days in both portions. Xpert positivity was 99 % concordant in ‘OM-S treated’ regardless of centrifugation.

Conclusions

OM-S is safe for sputum transportation. OM-S mixed sputum can be tested with Xpert and culture. Further studies can validate findings and assess cost-effectiveness.

查看原文本刊更多论文

OMNIgene。在环境温度下运输痰液用于结核病检测：孟加拉国的经验

OMNIgene的背景性能。痰液（OM-S）用于痰液运输。方法本探索性研究于2019年1月至12月在达卡的四家近距离医疗机构和一家远距离医疗机构进行。收集痰液阳性肺结核患者的痰液，将其分为“未经OM-S治疗”和“OM-S治疗”两部分，并于当天从近地点和从远地点通过快递运送到达卡检测实验室，进行涂片显微镜、培养和Xpert MTB/RIF （Xpert）检测。“OM-S处理”样品的子集在不离心的情况下用Xpert进行测试。比较各部分的试验结果。结果共纳入受试者444例（近点198例，远点246例）。所有的测试结果在近地点的两个部分具有可比性。对于远处部位，“OM-S处理”的涂片显微镜阳性降低了4.1%，Xpert在两个部分显示100%的一致性，“OM-S处理”的培养高于“OM-S未处理”(92.3% vs 89.4%；p = 0.288)。“OM-S处理”组的初级污染率低于“OM-S未处理”组(2.0% vs 9.8%；p & lt;0.05)。对于所有站点，两个部分的中位（IQR）培养阳性时间为35（28,42）天。无论离心与否，“OM-S处理”的专家阳性结果一致性为99%。结论som - s用于痰液运输是安全的。OM-S混合痰液可以用Xpert法和培养法检测。进一步的研究可以验证研究结果并评估成本效益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Tuberculosis and Other Mycobacterial Diseases Medicine-Pulmonary and Respiratory Medicine

CiteScore

4.00

自引率

5.00%

发文量

审稿时长

30 weeks

期刊介绍： Journal of Clinical Tuberculosis and Mycobacterial Diseases aims to provide a forum for clinically relevant articles on all aspects of tuberculosis and other mycobacterial infections, including (but not limited to) epidemiology, clinical investigation, transmission, diagnosis, treatment, drug-resistance and public policy, and encourages the submission of clinical studies, thematic reviews and case reports. Journal of Clinical Tuberculosis and Mycobacterial Diseases is an Open Access publication.