Preparation of African swine fever virus (ASFV) p30 monoclonal antibodies and identification of novel antigenic epitopes

IF 3 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jiajia Zhang , Kaili Zhang , Shaohua Sun , Dafu Deng , Ping He , Hanrong Lv , Sen Jiang , Wanglong Zheng , Nanhua Chen , Jinguo Gu , Jianfa Bai , Jianzhong Zhu
{"title":"Preparation of African swine fever virus (ASFV) p30 monoclonal antibodies and identification of novel antigenic epitopes","authors":"Jiajia Zhang ,&nbsp;Kaili Zhang ,&nbsp;Shaohua Sun ,&nbsp;Dafu Deng ,&nbsp;Ping He ,&nbsp;Hanrong Lv ,&nbsp;Sen Jiang ,&nbsp;Wanglong Zheng ,&nbsp;Nanhua Chen ,&nbsp;Jinguo Gu ,&nbsp;Jianfa Bai ,&nbsp;Jianzhong Zhu","doi":"10.1016/j.molimm.2025.07.009","DOIUrl":null,"url":null,"abstract":"<div><div>African swine fever (ASF) is a highly infectious disease caused by African swine fever virus (ASFV), with a mortality rate of up to 100 % for highly virulent strains. The ASFV p30 protein is encoded by the early transcriptional gene CP204L. As one of the structural proteins of ASFV, p30 is an ideal diagnostic antigen for ASF. Here, we first generated three monoclonal antibodies (mAbs) specific for p30 from immunized BALB/c mice via cell fusion, which were successfully applied in ELISA, Western blotting, and immunofluorescence assay. Second, two novel antigenic epitopes, <sup>169</sup>TIYGTPLKE<sup>177</sup> and <sup>111</sup>ETNECTSSFET<sup>121</sup> of p30 were identified using Western blotting with the three p30 mAbs. The two epitopes identified were highly conserved across genotypes I and/or II ASFVs. Third, an indirect ELISA based on epitope peptides of p30 was established to effectively detect antibodies during ASFV infection.</div></div>","PeriodicalId":18938,"journal":{"name":"Molecular immunology","volume":"185 ","pages":"Pages 18-26"},"PeriodicalIF":3.0000,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular immunology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0161589025001804","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

African swine fever (ASF) is a highly infectious disease caused by African swine fever virus (ASFV), with a mortality rate of up to 100 % for highly virulent strains. The ASFV p30 protein is encoded by the early transcriptional gene CP204L. As one of the structural proteins of ASFV, p30 is an ideal diagnostic antigen for ASF. Here, we first generated three monoclonal antibodies (mAbs) specific for p30 from immunized BALB/c mice via cell fusion, which were successfully applied in ELISA, Western blotting, and immunofluorescence assay. Second, two novel antigenic epitopes, 169TIYGTPLKE177 and 111ETNECTSSFET121 of p30 were identified using Western blotting with the three p30 mAbs. The two epitopes identified were highly conserved across genotypes I and/or II ASFVs. Third, an indirect ELISA based on epitope peptides of p30 was established to effectively detect antibodies during ASFV infection.
非洲猪瘟病毒(ASFV) p30单克隆抗体的制备及新型抗原表位的鉴定
非洲猪瘟(ASF)是由非洲猪瘟病毒(ASFV)引起的一种高度传染性疾病,高毒株的死亡率高达100% %。ASFV p30蛋白由早期转录基因CP204L编码。p30作为ASFV的结构蛋白之一,是一种理想的ASF诊断抗原。本研究首先通过细胞融合从免疫的BALB/c小鼠中获得3种特异性p30的单克隆抗体(mab),并成功应用于ELISA、Western blotting和免疫荧光分析。其次,采用Western blotting方法鉴定p30的两个新的抗原表位169TIYGTPLKE177和111ETNECTSSFET121。鉴定的两个表位在基因型I和/或II asfv中高度保守。第三,建立了一种基于p30表位肽的间接ELISA,可有效检测ASFV感染期间的抗体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Molecular immunology
Molecular immunology 医学-免疫学
CiteScore
6.90
自引率
2.80%
发文量
324
审稿时长
50 days
期刊介绍: Molecular Immunology publishes original articles, reviews and commentaries on all areas of immunology, with a particular focus on description of cellular, biochemical or genetic mechanisms underlying immunological phenomena. Studies on all model organisms, from invertebrates to humans, are suitable. Examples include, but are not restricted to: Infection, autoimmunity, transplantation, immunodeficiencies, inflammation and tumor immunology Mechanisms of induction, regulation and termination of innate and adaptive immunity Intercellular communication, cooperation and regulation Intracellular mechanisms of immunity (endocytosis, protein trafficking, pathogen recognition, antigen presentation, etc) Mechanisms of action of the cells and molecules of the immune system Structural analysis Development of the immune system Comparative immunology and evolution of the immune system "Omics" studies and bioinformatics Vaccines, biotechnology and therapeutic manipulation of the immune system (therapeutic antibodies, cytokines, cellular therapies, etc) Technical developments.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信