Serum anti-Müllerian hormone (AMH) concentrations in pregnant and lactating dogs

IF 2.5 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-07-10 DOI:10.1016/j.theriogenology.2025.117571

Sebastian P. Arlt , Janna Hinderer , Lisa Riege , Johanna Leber , Elisabeth Müller , Corinna Weber , Beate Walter

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引用次数: 0

Abstract

Measurement of Anti-Müllerian hormone (AMH) in female dogs is becoming increasingly important in clinical practice. It has been shown earlier that concentrations vary throughout the estrous cycle. AMH concentrations are known to be high during proestrus and estrus, then decrease during the luteal phase and anestrus.

Aim of this study was to investigate variations during pregnancy and lactation. Therefore, blood samples were analyzed from 40 pregnant and 19 non-pregnant privately owned dogs of various breeds. One sample was collected from each dog during estrus, three samples during pregnancy, one at three weeks in lactation, and one after weaning. The measured concentrations were compared with those from the non-pregnant dogs, from which samples were collected at the same corresponding time points.

AMH concentration in pregnant dogs was highest in estrus (0.63 ng/ml, quartiles 0.40 and 0.87) and remained relatively high during pregnancy. At sampling during lactation the median concentration was significantly lower compared with all other time points (median 0.19 ng/ml, quartiles 0.14 and 0.26). After weaning, AMH increased again to 0.30 ng/ml (quartiles 0.24 and 0.50). During late pregnancy, lactation and after weaning, the AMH concentration was lower in the pregnant dogs compared with the non-pregnant ones. In addition, the significant decline of AMH concentration in pregnant dogs during lactation was not present in the non-pregnant dogs.

It can be hypothesized that higher prolactin concentrations may reduce AMH synthesis by inhibiting the secretion of GnRH, FSH, and LH in mammals. In our study we found moderate negative correlation between bodyweight or the number of suckling puppies and AMH concentration. However, since no assay was available, prolactin concentrations were not measured. These findings indicate that pregnancy and lactation status should be considered when interpreting AMH concentrations in clinical practice.

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妊娠和哺乳期犬血清抗勒氏杆菌激素（AMH）浓度

雌性犬抗勒氏杆菌激素（AMH）的测定在临床实践中越来越重要。早些时候已经证明，在整个发情周期中，浓度是不同的。已知AMH浓度在发情前期和发情期间较高，然后在黄体期和发情期间降低。本研究的目的是调查妊娠和哺乳期的变化。因此，我们分析了40只怀孕和19只未怀孕的不同品种的私人狗的血液样本。每只狗在发情期采集一份样本，在怀孕期间采集三份样本，在哺乳期三周采集一份样本，在断奶后采集一份样本。将测量到的浓度与未怀孕犬的浓度进行比较，未怀孕犬的样本在相同的相应时间点收集。怀孕犬AMH浓度在发情期最高（0.63 ng/ml，四分位数分别为0.40和0.87），在怀孕期间保持较高水平。在哺乳期采样时，中位数浓度显著低于所有其他时间点（中位数0.19 ng/ml，四分位数0.14和0.26）。断奶后，AMH再次升高至0.30 ng/ml（四分位数0.24和0.50）。妊娠后期、哺乳期和断奶后，妊娠犬的AMH浓度均低于未妊娠犬。此外，怀孕犬在哺乳期间AMH浓度的显著下降在未怀孕犬中不存在。可以推测，较高的催乳素浓度可能通过抑制哺乳动物GnRH、FSH和LH的分泌来减少AMH的合成。在我们的研究中，我们发现体重或哺乳幼犬数量与AMH浓度呈中度负相关。然而，由于没有可用的分析，催乳素浓度没有测量。这些发现表明，在临床实践中解释AMH浓度时应考虑妊娠和哺乳状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.