Low molecular weight Astragalus membranaceus polysaccharides alleviates dextran sulfate sodium-induced colitis in mice

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-07-10 DOI:10.1016/j.carbpol.2025.124050

Meng Ye , Menghan Fan , Yi Zhao , Feng Wang , Xiaobing Yang , Wenbing Yao , Xiangdong Gao , Juping Yu , Wei Liu

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Abstract

Inflammatory bowel disease (IBD) treatment is challenged by limited efficacy and side effects of current therapies. While Astragalus membranaceus polysaccharides (APS) show anti-inflammatory potential, their structure-activity relationship is unclear. This study compared the therapeutic effects of the linear α-1,4-glucan APS-G2 and the branched-chain APS-A1, enzymatically derived APS oligosaccharides. Structure-function analysis revealed that the homogeneous α-1,4-glucan backbone of APS-G2 is essential for superior bioactivity, contrasting with the diminished efficacy of the heterogeneous, branched APS-A1. APS-G2 significantly suppressed pro-inflammatory cytokines, elevated anti-inflammatory mediators, and improved intestinal barrier integrity by preventing DSS-induced apoptosis of intestinal epithelial cells and promoting the expression of tight junction proteins. The underlying mechanisms involve coordinated regulation of the SIRT1/PGC-1α/NF-κB pathway and FXR-mediated signaling. These findings elucidate the structure-function relationships of APS derivatives and suggest that enzymatic engineering can enhance the therapeutic potential of polysaccharides for IBD management.

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低分子量黄芪多糖减轻硫酸葡聚糖钠所致小鼠结肠炎

炎症性肠病（IBD）的治疗受到目前治疗方法有限的疗效和副作用的挑战。黄芪多糖（Astragalus membraneaceus polysaccharides， APS）具有抗炎活性，但其构效关系尚不清楚。本研究比较了线性α-1,4-葡聚糖APS- g2和支链APS- a1的治疗效果。结构-功能分析表明，APS-G2具有均匀的α-1,4-葡聚糖主链，具有较好的生物活性，而非均匀的支链APS-A1具有较低的生物活性。APS-G2通过阻止dss诱导的肠上皮细胞凋亡和促进紧密连接蛋白的表达，显著抑制促炎细胞因子，升高抗炎介质，改善肠屏障完整性。潜在的机制涉及SIRT1/PGC-1α/NF-κB通路和fxr介导的信号通路的协调调节。这些发现阐明了多糖衍生物的结构-功能关系，并表明酶工程可以增强多糖治疗IBD的潜力。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.