Marjan Ghiyasvand, Ardeshir Hesampour, Hossein Dabiri, Javad Arasteh
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引用次数: 0
Abstract
Background: Colorectal cancer is one of the deadliest cancers with poor prognosis and limited treatment options. Microbiota and their metabolites, including toxins, have been shown to possess anti-tumor properties. Outer membrane vesicles (OMVs) from Escherichia coli may trigger inflammation in intestinal cells. We aimed to explore the anti-cancer effects of E. coli-derived OMVs on colorectal cancer cells by promoting apoptosis and pyroptosis, particularly through the over-expression of GSDMD in the CT-26 cell line.
Methods: In 2022, five non-replicating probiotic strains of E. coli were obtained from the Histogenotech Company in Tehran, and their OMVs were isolated and characterized. The CT-26 cell line was used to assess cell toxicity and the apoptotic effects of E. coli-derived OMVs. Real-time PCR and western blotting were performed to measure the expression of apoptosis and pyroptosis markers (GSDMD, NLRP3, and ROCK).
Results: The E. coli strains produced nanovesicles (5-200 nm in diameter). OMVs significantly reduced the viability of CT-26 cells and induced apoptosis at a concentration of 10 µg. The expression of GSDMD and NLRP3 was increased, while ROCK expression was downregulated in treated colorectal cells.
Conclusion: E. coli-derived OMVs can trigger inflammatory processes and induce negative effects on colorectal cancer cells. Further research is needed to evaluate their potential for colorectal cancer treatment.
期刊介绍:
Iranian Journal of Public Health has been continuously published since 1971, as the only Journal in all health domains, with wide distribution (including WHO in Geneva and Cairo) in two languages (English and Persian). From 2001 issue, the Journal is published only in English language. During the last 41 years more than 2000 scientific research papers, results of health activities, surveys and services, have been published in this Journal. To meet the increasing demand of respected researchers, as of January 2012, the Journal is published monthly. I wish this will assist to promote the level of global knowledge. The main topics that the Journal would welcome are: Bioethics, Disaster and Health, Entomology, Epidemiology, Health and Environment, Health Economics, Health Services, Immunology, Medical Genetics, Mental Health, Microbiology, Nutrition and Food Safety, Occupational Health, Oral Health. We would be very delighted to receive your Original papers, Review Articles, Short communications, Case reports and Scientific Letters to the Editor on the above mentioned research areas.