{"title":"Optimization, Characterization and Antibacterial Activity of Copper Nanoparticles Biosynthesized Using <i>Pantoea agglomerans</i> CPHN2.","authors":"Simran Rani, Pradeep Kumar, Priyanka Dahiya, Aditi Mehta, Amita Suneja Dang, Pooja Suneja","doi":"10.1007/s12088-025-01455-2","DOIUrl":null,"url":null,"abstract":"<p><p>'Green' synthesis of nanoparticles from microorganisms and plants is a sustainable alternative to the conventional chemo-physical methods because of its environment-friendliness. Microorganisms take up extracellular and intracellular pathways to manufacture nanoparticles. This study was aimed at optimization of the parameters influencing the biosynthesis of Cu/CuONPs (Copper/Copper oxide nanoparticles) by <i>Pantoea agglomerans</i> CPHN2 using OFAT (One-factor-at-a-time) approach. The optimum biotransformation was achieved at 5 mM concentration of CuSO<sub>4</sub>, 32 h incubation time, 6 h reaction time, equal mixing ratio of supernatant and CuSO<sub>4</sub>, 7 pH, and 23 °C temperature. Biosynthesized Cu/CuONPs along with those formed chemically, were characterized using UV-Visible spectrophotometer, resulting in a characteristic peak between 550 and 650 nm. Dynamic light scattering (DLS) reported that Z-average and Zeta potential of Cu/CuONPs were 291.9 nm and - 21 mV for biosynthesized and 179 nm and - 6.49 mV for chemosynthesized NPs. HR-TEM (High Resolution-Transmission Electron Microscopy) revealed hexagonal NPs in size range of 5-35 nm. In addition, FTIR (Fourier Transform Infrared) spectrum elucidated different peaks attributed to the Cu/CuONPs, alkynes, O-H, and N-H bonds, of the molecules involved in synthesis and stabilization of biosynthesized NPs. Antimicrobial assay conducted in the presence of 100 µg/ml solution of biosynthesized Cu/CuONPs led to 66.01, 68.31, and 55.73%, inhibition of <i>Staphylococcus aureus</i> growth at 7, 14, and 21 h, respectively, whereas chemosynthesized Cu/CuONPs had negligible impact. The biosynthesized NPs also exhibited 5.48-fold increase in inhibitory activity than chemosynthesized NPs on <i>Bacillus subtilis</i> growth. The results suggests that biosynthesized Cu/CuONPs can serve as ecofriendly and economical method for managing the infections led by gram-positive bacteria.</p><p><strong>Graphical abstract: </strong></p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s12088-025-01455-2.</p>","PeriodicalId":13316,"journal":{"name":"Indian Journal of Microbiology","volume":"65 2","pages":"1345-1356"},"PeriodicalIF":2.1000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246322/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12088-025-01455-2","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/8 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
'Green' synthesis of nanoparticles from microorganisms and plants is a sustainable alternative to the conventional chemo-physical methods because of its environment-friendliness. Microorganisms take up extracellular and intracellular pathways to manufacture nanoparticles. This study was aimed at optimization of the parameters influencing the biosynthesis of Cu/CuONPs (Copper/Copper oxide nanoparticles) by Pantoea agglomerans CPHN2 using OFAT (One-factor-at-a-time) approach. The optimum biotransformation was achieved at 5 mM concentration of CuSO4, 32 h incubation time, 6 h reaction time, equal mixing ratio of supernatant and CuSO4, 7 pH, and 23 °C temperature. Biosynthesized Cu/CuONPs along with those formed chemically, were characterized using UV-Visible spectrophotometer, resulting in a characteristic peak between 550 and 650 nm. Dynamic light scattering (DLS) reported that Z-average and Zeta potential of Cu/CuONPs were 291.9 nm and - 21 mV for biosynthesized and 179 nm and - 6.49 mV for chemosynthesized NPs. HR-TEM (High Resolution-Transmission Electron Microscopy) revealed hexagonal NPs in size range of 5-35 nm. In addition, FTIR (Fourier Transform Infrared) spectrum elucidated different peaks attributed to the Cu/CuONPs, alkynes, O-H, and N-H bonds, of the molecules involved in synthesis and stabilization of biosynthesized NPs. Antimicrobial assay conducted in the presence of 100 µg/ml solution of biosynthesized Cu/CuONPs led to 66.01, 68.31, and 55.73%, inhibition of Staphylococcus aureus growth at 7, 14, and 21 h, respectively, whereas chemosynthesized Cu/CuONPs had negligible impact. The biosynthesized NPs also exhibited 5.48-fold increase in inhibitory activity than chemosynthesized NPs on Bacillus subtilis growth. The results suggests that biosynthesized Cu/CuONPs can serve as ecofriendly and economical method for managing the infections led by gram-positive bacteria.
Graphical abstract:
Supplementary information: The online version contains supplementary material available at 10.1007/s12088-025-01455-2.
期刊介绍：
Indian Journal of Microbiology is the official organ of the Association of Microbiologists of India (AMI). It publishes full-length papers, short communication reviews and mini reviews on all aspects of microbiological research, published quarterly (March, June, September and December). Areas of special interest include agricultural, food, environmental, industrial, medical, pharmaceutical, veterinary and molecular microbiology.
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