Genomic Diversity and Antimicrobial Resistance of Acinetobacter sp.-A Pan Genome Analysis.

IF 2.1 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Indian Journal of Microbiology Pub Date : 2025-06-01 Epub Date: 2024-10-22 DOI:10.1007/s12088-024-01411-6

R Shobana Manoharan, K Raghavan, M Jayalakshmi

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Abstract

Acinetobacter baumannii has been associated with severe clinically acquired infections. The prevalence of non-A. baumannii-linked diseases is now equivalent to that of A. baumannii-associated illnesses. Hence, this study intends to explore the genomic linkage of virulence and antibiotic resistance amongst the five Acinetobacter spp. (A. baumannii, A. haemolyticus, A. johnsonni, and A. nosocomialis) using pan genome analysis. The results revealed open pan genome in Acinetobacter sp. with increase in number of additional genes in pan genome. The resistance gene identifier revealed that A. baumannii was resistant to macrolides, fluoroquinolone, lincosamide, carbapenem, cephalosporin, tetracycline, rifamycin, diaminopyrimidine, phenicol antibiotics, and penem. Non-A. baumannii sp. A. haemolyticus had the Oxa beta lactamase gene (Oxa) with resistance to carbapenem, cephalosporin, penem. A. johnsonii had no perfect hits for resistance but showed weak similarity to Oxa. A. nosocomialis had resistance genes to fluoroquinoids. The prevalence of Acinetobacter baumannii AbaQ gene was found with A. calcoaceticus and A. nosocomialis sequences used in this study supporting the transformation of resistance genes between Acinetobacter sp. This comparative research underlines the need of differentiating between distinct Acinetobacter sp. infections with their genetic profile for accurate diagnosis and management based on their anti microbial resistance.

Supplementary information: The online version contains supplementary material available at 10.1007/s12088-024-01411-6.

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不动杆菌基因组多样性与抗微生物药物耐药性分析。

鲍曼不动杆菌与严重的临床获得性感染有关。非a。鲍曼原虫相关疾病现在等同于鲍曼原虫相关疾病。因此，本研究拟利用全基因组分析方法探讨鲍曼不动杆菌、溶血不动杆菌、约翰逊不动杆菌和医院不动杆菌五种不动杆菌的毒力和耐药性的基因组联系。结果表明，不动杆菌的pan基因组是开放的，pan基因组中的附加基因数量增加。耐药基因鉴定显示鲍曼不动杆菌对大环内酯类、氟喹诺酮类、林可沙胺类、碳青霉烯类、头孢菌素类、四环素类、利福霉素类、二氨基嘧啶类、酚类抗生素和培南类耐药。许可。溶血性鲍曼原虫携带Oxa -内酰胺酶基因（Oxa），对碳青霉烯类、头孢菌素类、培南类耐药。jonsonii在抗性方面没有完美的匹配，但与Oxa的相似性较弱。医院甲螨对氟喹酮类药物有耐药基因。本研究发现，鲍曼不动杆菌AbaQ基因在钙酸不动杆菌和医院不动杆菌中普遍存在，这支持了不动杆菌之间耐药基因的转化。这一比较研究强调了区分不同不动杆菌感染的基因特征，以便根据其抗微生物耐药性进行准确诊断和管理的必要性。补充资料：在线版本包含补充资料，网址为10.1007/s12088-024-01411-6。

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来源期刊

Indian Journal of Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY

CiteScore

6.00

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： Indian Journal of Microbiology is the official organ of the Association of Microbiologists of India (AMI). It publishes full-length papers, short communication reviews and mini reviews on all aspects of microbiological research, published quarterly (March, June, September and December). Areas of special interest include agricultural, food, environmental, industrial, medical, pharmaceutical, veterinary and molecular microbiology.