Single-cell analysis reveals the spatiotemporal effects of long-term electromagnetic field exposure on the liver.

IF 4.6 2区生物学 Q2 CELL BIOLOGY

Frontiers in Cell and Developmental Biology Pub Date : 2025-06-27 eCollection Date: 2025-01-01 DOI:10.3389/fcell.2025.1579121

Mingming Zhang, Zhichun Lv, Lingping Zhao, Quan Zeng, Yunqiang Wu, Junnian Zhou, Jiafei Xi, Xuetao Pei, Haiyang Wang, Changyan Li, Wen Yue

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引用次数: 0

Abstract

Introduction: Artificial electromagnetic fields (EMFs) can impair the functions of several organs. The impact of long-term artificial EMF on the liver, the synthetic and metabolic center of the body, has become concerning. The aim of this study was to systematically evaluate the effect of long-term EMF exposure on the liver.

Methods: Mice were exposed to 2.45 GHz EMF daily for up to 5 months, and serum liver function test, lipidomic analysis, and histological analysis were performed to detect the general impact of EMF on the liver. Furthermore, EMF-induced liver transcriptome variations were investigated using single-cell RNA sequencing and a spatiotemporally resolved analysis.

Results: Different hepatic cells exhibited diverse sensitivities and response patterns. Notably, hepatocytes, endothelial cells, and monocytes showed higher sensitivity to electromagnetic radiation, with their lipid metabolic functions, immune regulation functions, and intrinsic functions disturbed, respectively. Moreover, transcriptomic alterations were predominantly observed in the hepatocytes and endothelial cells in peri-portal regions, suggesting a zonation-related sensitivity to EMF within the liver.

Conclusion: Our study provided a spatiotemporal visualization of EMF-induced alterations in hepatic cells, which ultimately elucidated the biological effects of EMF exposure.

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单细胞分析揭示了长期电磁场暴露对肝脏的时空影响。

导言：人工电磁场（emf）会损害几个器官的功能。长期人工电磁场对人体合成和代谢中心肝脏的影响已引起人们的关注。本研究的目的是系统地评估长期电磁场暴露对肝脏的影响。方法：将小鼠每天暴露于2.45 GHz电磁场长达5个月，进行血清肝功能测试、脂质组学分析和组织学分析，以检测电磁场对肝脏的一般影响。此外，使用单细胞RNA测序和时空分辨分析研究了emf诱导的肝脏转录组变异。结果：不同的肝细胞表现出不同的敏感性和反应模式。值得注意的是，肝细胞、内皮细胞和单核细胞对电磁辐射表现出更高的敏感性，其脂质代谢功能、免疫调节功能和内在功能分别受到干扰。此外，转录组改变主要在肝细胞和门静脉周围的内皮细胞中观察到，这表明肝脏内对EMF具有分区相关的敏感性。结论：我们的研究提供了电磁场诱导的肝细胞变化的时空可视化，最终阐明了电磁场暴露的生物学效应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Cell and Developmental Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

9.70

自引率

3.60%

发文量

2531

审稿时长

12 weeks

期刊介绍： Frontiers in Cell and Developmental Biology is a broad-scope, interdisciplinary open-access journal, focusing on the fundamental processes of life, led by Prof Amanda Fisher and supported by a geographically diverse, high-quality editorial board. The journal welcomes submissions on a wide spectrum of cell and developmental biology, covering intracellular and extracellular dynamics, with sections focusing on signaling, adhesion, migration, cell death and survival and membrane trafficking. Additionally, the journal offers sections dedicated to the cutting edge of fundamental and translational research in molecular medicine and stem cell biology. With a collaborative, rigorous and transparent peer-review, the journal produces the highest scientific quality in both fundamental and applied research, and advanced article level metrics measure the real-time impact and influence of each publication.