{"title":"Regulatory Role and Mechanism of lncRNA RNF217-AS1 in the Proliferation and Migration of Esophageal Cancer Cells.","authors":"Jie Liang, Xiaoli Niu, Gaoyan Wang, Minghui Wang","doi":"10.2147/CMAR.S515036","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aims to explore the effect of the long non-coding RNA (lncRNA) RNF217-AS1 on the proliferation and migration of esophageal cancer cells, and to uncover the molecular mechanisms through which RNF217-AS1 regulates these processes.</p><p><strong>Methods: </strong>The expression of RNF217-AS1 was measured in esophageal cancer cell lines (EC9706, Ecal09, KYSE-510, and TE-13) and immortalized esophageal epithelial HET-1 A cells using RT-qPCR. KYSE-510 cells were transfected with si-NC or si-RNF217-AS1 plasmids. Colony formation assays were used to assess cell proliferation, while migration ability was evaluated using scratch assays. A dual-luciferase reporter system was employed to verify the interaction between RNF217-AS1 and miR-377-3p. The expression of miR-377-3p and key proteins related to cell migration and epithelial-to-mesenchymal transition (EMT) were detected by RT-qPCR and Western blot.</p><p><strong>Results: </strong>RNF217-AS1 expression was significantly upregulated in esophageal cancer cells compared to HET-1 A cells (P<0.01). Downregulation of RNF217-AS1 in KYSE-510 and Eca109 cells led to a reduction in cell proliferation and migration (P<0.01). The dual-luciferase assay confirmed the interaction between RNF217-AS1 and miR-377-3p (P<0.01). miR-377-3p expression was elevated in the si-RNF217-AS1 group compared to the si-NC group (P<0.01). Furthermore, the protein levels of HOXA1, fibronectin, and FOXC2 were downregulated, while GRHL2 and E-cadherin expressions were increased in the si-RNF217-AS1 group (P<0.01).</p><p><strong>Conclusion: </strong>RNF217-AS1 is upregulated in esophageal cancer cells, and its downregulation inhibits the proliferation, migration and EMT of esophageal cancer cells by regulating the miR-377-3p/HOXA1 axis.</p>","PeriodicalId":9479,"journal":{"name":"Cancer Management and Research","volume":"17 ","pages":"1329-1337"},"PeriodicalIF":2.6000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254194/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cancer Management and Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2147/CMAR.S515036","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: This study aims to explore the effect of the long non-coding RNA (lncRNA) RNF217-AS1 on the proliferation and migration of esophageal cancer cells, and to uncover the molecular mechanisms through which RNF217-AS1 regulates these processes.
Methods: The expression of RNF217-AS1 was measured in esophageal cancer cell lines (EC9706, Ecal09, KYSE-510, and TE-13) and immortalized esophageal epithelial HET-1 A cells using RT-qPCR. KYSE-510 cells were transfected with si-NC or si-RNF217-AS1 plasmids. Colony formation assays were used to assess cell proliferation, while migration ability was evaluated using scratch assays. A dual-luciferase reporter system was employed to verify the interaction between RNF217-AS1 and miR-377-3p. The expression of miR-377-3p and key proteins related to cell migration and epithelial-to-mesenchymal transition (EMT) were detected by RT-qPCR and Western blot.
Results: RNF217-AS1 expression was significantly upregulated in esophageal cancer cells compared to HET-1 A cells (P<0.01). Downregulation of RNF217-AS1 in KYSE-510 and Eca109 cells led to a reduction in cell proliferation and migration (P<0.01). The dual-luciferase assay confirmed the interaction between RNF217-AS1 and miR-377-3p (P<0.01). miR-377-3p expression was elevated in the si-RNF217-AS1 group compared to the si-NC group (P<0.01). Furthermore, the protein levels of HOXA1, fibronectin, and FOXC2 were downregulated, while GRHL2 and E-cadherin expressions were increased in the si-RNF217-AS1 group (P<0.01).
Conclusion: RNF217-AS1 is upregulated in esophageal cancer cells, and its downregulation inhibits the proliferation, migration and EMT of esophageal cancer cells by regulating the miR-377-3p/HOXA1 axis.
期刊介绍：
Cancer Management and Research is an international, peer reviewed, open access journal focusing on cancer research and the optimal use of preventative and integrated treatment interventions to achieve improved outcomes, enhanced survival, and quality of life for cancer patients. Specific topics covered in the journal include:
◦Epidemiology, detection and screening
◦Cellular research and biomarkers
◦Identification of biotargets and agents with novel mechanisms of action
◦Optimal clinical use of existing anticancer agents, including combination therapies
◦Radiation and surgery
◦Palliative care
◦Patient adherence, quality of life, satisfaction
The journal welcomes submitted papers covering original research, basic science, clinical & epidemiological studies, reviews & evaluations, guidelines, expert opinion and commentary, and case series that shed novel insights on a disease or disease subtype.
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