Taha Ghantabpour, Mansoureh Soleimani, Reza Ahadi, Fariba Karimzadeh, Alireza Moradi
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引用次数: 0
Abstract
Introduction: Epilepsy is characterized by recurrent seizures associated with cognitive, mental, and social issues. Exercise has been well known as a non-pharmacological or complementary remedy that reduces the effective dose and side effects of pharmacological therapies. Orexin signaling pathway and brain-derived neurotrophic factor (BDNF) have an essential role in the pathogenesis of epilepsy. In this study, we investigated the effect of exercise on the modulation of the orexin-A (OXA) and BDNF signaling pathways in epileptic rats.
Methods: Male Wistar rats were divided into 5 groups: Normal saline (NS), seizure, physical activity (PA), PA + pentylenetetrazol (PTZ), and PA-PTZ. Assessment of seizure behaviors was done 30 min after each PTZ injection in the seizure, PA+PTZ, and PA-PTZ groups. Seizure behavior score (SBS) was monitored in seizure, PA+PTZ, and PA-PTZ. The expression of the OXA and BDNF in the CA1, CA3, and cortex was assayed by immunohistochemistry staining. The correlations between the OXA and BDNF were evaluated in the study groups.
Results: SBC was reduced in the epileptic rats that had exercised. Seizure and PA increased the OXA expression in the seizure and PA groups. Compared to the seizure group, the OXA expression decreased in the CA1 and CA3 of the PA+PTZ, PA-PTZ, and cortex of the PA+PTZ group. OXA was up-expressed in the PA-PTZ group compared to the PA+PTZ group. Seizure decreased the BDNF expression in the seizure group compared to the NS group. PA elevated the BDNF expression in the CA1, CA3, and cortex of the PA group. BDNF was up-expressed in the cortex of the PA+PTZ and the CA1, CA3, and cortex of PA-PTZ. BDNF expression increased in the CA1 and CA3 of the PA-PTZ compared to the PA+PTZ group. There was a significant correlation between the OXA and BDNF expression in the CA1, CA3, and cortex of the NS and seizure groups and the CA1 and cortex of the PA group.
Conclusion: Our results indicate that PA had an amelioration effect on the severity of the seizure. Our findings suggest that the effect of PA on seizure might not arise from the interaction of the OXA and BDNF expression in epileptic rats.
癫痫的特点是反复发作，并伴有认知、精神和社会问题。众所周知，运动是一种非药物或补充疗法，可以减少药物治疗的有效剂量和副作用。食欲素信号通路和脑源性神经营养因子（BDNF）在癫痫发病中起重要作用。在这项研究中，我们研究了运动对癫痫大鼠食欲素- a （OXA）和BDNF信号通路的调节作用。方法：雄性Wistar大鼠分为生理盐水组（NS）、癫痫组（PA）、体力活动组（PA）、PA +戊四唑组（PTZ）和PA-PTZ组（PA -PTZ）。癫痫发作组、PA+PTZ组和PA-PTZ组在每次注射PTZ后30分钟评估癫痫发作行为。监测癫痫发作、PA+PTZ和PA-PTZ患者的发作行为评分（SBS）。免疫组化染色检测CA1、CA3和皮质中OXA和BDNF的表达。在研究组中评估OXA和BDNF之间的相关性。结果：运动后癫痫大鼠SBC明显减少。发作组和PA组OXA表达升高。与癫痫发作组相比，PA+PTZ、PA-PTZ和PA+PTZ皮层CA1、CA3表达降低。与PA+PTZ组相比，PA-PTZ组OXA表达升高。与NS组相比，癫痫发作组BDNF表达降低。PA组BDNF在CA1、CA3和皮质中的表达升高。BDNF在PA+PTZ和PA-PTZ的CA1、CA3、皮质中表达上调。与PA+PTZ组相比，PA-PTZ的CA1和CA3中BDNF表达增加。NS组和癫痫组CA1、CA3和皮质以及PA组CA1和皮质中OXA和BDNF的表达有显著相关性。结论：PA对癫痫发作的严重程度有改善作用。我们的研究结果表明，PA对癫痫大鼠癫痫发作的影响可能不是由OXA和BDNF表达的相互作用引起的。
期刊介绍：
BCN is an international multidisciplinary journal that publishes editorials, original full-length research articles, short communications, reviews, methodological papers, commentaries, perspectives and “news and reports” in the broad fields of developmental, molecular, cellular, system, computational, behavioral, cognitive, and clinical neuroscience. No area in the neural related sciences is excluded from consideration, although priority is given to studies that provide applied insights into the functioning of the nervous system. BCN aims to advance our understanding of organization and function of the nervous system in health and disease, thereby improving the diagnosis and treatment of neural-related disorders. Manuscripts submitted to BCN should describe novel results generated by experiments that were guided by clearly defined aims or hypotheses. BCN aims to provide serious ties in interdisciplinary communication, accessibility to a broad readership inside Iran and the region and also in all other international academic sites, effective peer review process, and independence from all possible non-scientific interests. BCN also tries to empower national, regional and international collaborative networks in the field of neuroscience in Iran, Middle East, Central Asia and North Africa and to be the voice of the Iranian and regional neuroscience community in the world of neuroscientists. In this way, the journal encourages submission of editorials, review papers, commentaries, methodological notes and perspectives that address this scope.
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