Application of Cryopreserved Decellularized Human Amniotic Membrane for Healing of Treatment-resistant Cesarean Section Wounds: A Case Series.

Abstract

Considering the increase in cesarean surgeries in recent years and, therefore, the complications related to this type of surgical wound, attention has been drawn to treatment methods such as biological wound dressings. In this case series, the authors describe the treatment of infected cesarean wounds with impaired healing in 4 women. For this aim, human placentas were collected under sterile conditions and amniotic membranes were separated from the placenta. To decrease immunogenic response, the membrane was decellularized using 1% Triton-x-100, 1 mmol/L EDTA, and Trypsin 0.05. Then, using glycerol and Dulbecco Modified Eagle Medium, the decellularized amniotic membrane (dAM) was frozen for 3 months at -80 °C. After this period, the dAM was used to dress the wounds of the 4 patients. All patients treated with dAM achieved complete wound healing, indicating that dAM may be an effective treatment method for cesarean section wounds, especially those prone to complications.