Acupuncture blocks the STAT3-MMP-2 pathway in a guinea pig model of lens-induced myopia.

IF 2.6 3区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Acupuncture in Medicine Pub Date : 2025-08-01 Epub Date: 2025-07-13 DOI:10.1177/09645284251345847

Yu-Hui Zhao, Xiu-Zhen Lu, Ji-Cun Qian, Wen-Hui Li, Qiu-Xin Wu, Xing-Bo He, Hua-Yue Sun, Zhao-Hui Yang, Qi Hao, Zong-Long Hao, Ji-Ke Song, Qing-Mei Tian, Hong-Sheng Bi

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引用次数: 0

Abstract

Objective: To investigate the effects of manual acupuncture (MA) on the signal transduction and activator of transcription (STAT)3 - matrix metalloproteinase (MMP)-2 pathway in a guinea pig model of lens-induced myopia (LIM).

Methods: A total of 36 male pigmented guinea pigs (aged 2 weeks) were randomly assigned to the normal control (NC) (n = 12), LIM (n = 12) and LIM + MA (n = 12) groups. The NC group received no intervention. To create myopia, the right eyes of the LIM and LIM + MA groups were covered with -6.00 D lenses. The LIM + MA group received 30 min of acupuncture stimulation daily at Taiyang and LI4. The effect of LIM modelling and MA on ocular measurements, expression of STAT3, phosphorylated (p)-STAT3, MMP-2 and tissue inhibitor of MMP-2 (TIMP)-2 expression (as well as histopathology, morphology and immunohistochemistry of the guinea pigs' eyes) were evaluated.

Results: Compared with the NC group, the LIM group showed increased expression of STAT3, p-STAT3 and MMP-2, and decreased expression of TIMP-2, as well as an increase in ocular measurements. The eye tissue morphology was disrupted and fibres became thinner in the LIM group. Compared to the LIM group, the LIM + MA group exhibited a noteworthy decline in the expression of STAT3, p-STAT3 and MMP-2, a significant increase in TIMP-2, and a decrease in ocular measurements and tissue morphology, which were similar to that of the NC group.

Conclusion: Acupuncture has the potential to decrease STAT3 activation and block the STAT3-MMP-2 pathway in the ocular tissue of guinea pigs with experimental LIM, thereby delaying the development of myopia.

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在豚鼠透镜性近视模型中，针灸阻断STAT3-MMP-2通路。

目的：探讨手针刺（MA）对豚鼠晶状体性近视（LIM）信号转导及转录激活因子（STAT）3 -基质金属蛋白酶(MMP)-2通路的影响。方法：36只2周龄雄性色素豚鼠随机分为正常对照组（NC）（n = 12）、LIM组（n = 12）和LIM + MA组（n = 12）。NC组不进行干预。为了制造近视，LIM组和LIM + MA组右眼覆盖-6.00 D晶状体。LIM + MA组分别在太阳和LI4处进行每日30 min的针刺刺激。评估LIM模型和MA对豚鼠眼部测量、STAT3、磷酸化(p)-STAT3、MMP-2和MMP-2组织抑制剂(TIMP)-2表达的影响（以及组织病理学、形态学和免疫组化）。结果：与NC组比较，LIM组STAT3、p-STAT3、MMP-2表达升高，TIMP-2表达降低，眼部测量值升高。LIM组眼组织形态被破坏，纤维变薄。与LIM组相比，LIM + MA组的STAT3、p-STAT3和MMP-2的表达明显下降，TIMP-2的表达明显升高，眼部测量值和组织形态下降，与NC组相似。结论：针刺有可能降低实验性LIM豚鼠眼组织中STAT3的激活，阻断STAT3- mmp -2通路，从而延缓近视的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

4.70

自引率

4.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.