{"title":"Standard in vitro co-culture conditions for studying inter-organ interactions between liver, breast cancer, and heart models","authors":"Gabriela Ulanowicz, Malgorzata Gluchowska, Agnieszka Zuchowska","doi":"10.1016/j.cbi.2025.111659","DOIUrl":null,"url":null,"abstract":"<div><div>Interactions between organs are crucial for the proper functioning of the body and the maintaining of homeostasis, so their reflection in <em>in vitro</em> studies is necessary. These interactions occur, among other things, through secretory compounds secreted by cells that, after entering the bloodstream, affect other organs. Therefore, <em>in vitro</em> studies should not be limited to recreating single tissue models, such as in the form of three-dimensional structures for testing cancer therapies or disease modeling. In the present study, the focus was on reproducing organ-to-organ communication <em>in vitro</em> using three-dimensional cultures of liver, breast cancer and heart models. The goal was to investigate whether the metabolites produced by these models, released into the culture medium, affect the functioning of the other models. The applied approach made it possible to observe significant differences in the metabolic activity of cell aggregates, the level of glucose consumption and the expression of selected proteins.</div></div>","PeriodicalId":274,"journal":{"name":"Chemico-Biological Interactions","volume":"419 ","pages":"Article 111659"},"PeriodicalIF":5.4000,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemico-Biological Interactions","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009279725002893","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Interactions between organs are crucial for the proper functioning of the body and the maintaining of homeostasis, so their reflection in in vitro studies is necessary. These interactions occur, among other things, through secretory compounds secreted by cells that, after entering the bloodstream, affect other organs. Therefore, in vitro studies should not be limited to recreating single tissue models, such as in the form of three-dimensional structures for testing cancer therapies or disease modeling. In the present study, the focus was on reproducing organ-to-organ communication in vitro using three-dimensional cultures of liver, breast cancer and heart models. The goal was to investigate whether the metabolites produced by these models, released into the culture medium, affect the functioning of the other models. The applied approach made it possible to observe significant differences in the metabolic activity of cell aggregates, the level of glucose consumption and the expression of selected proteins.
期刊介绍:
Chemico-Biological Interactions publishes research reports and review articles that examine the molecular, cellular, and/or biochemical basis of toxicologically relevant outcomes. Special emphasis is placed on toxicological mechanisms associated with interactions between chemicals and biological systems. Outcomes may include all traditional endpoints caused by synthetic or naturally occurring chemicals, both in vivo and in vitro. Endpoints of interest include, but are not limited to carcinogenesis, mutagenesis, respiratory toxicology, neurotoxicology, reproductive and developmental toxicology, and immunotoxicology.