Design and Characterization of PAA/CHI/Triclosan Multilayer Films with Long-Term Antibacterial Activity.

IF 4.7 3区工程技术 Q1 POLYMER SCIENCE

Polymers Pub Date : 2025-06-27 DOI:10.3390/polym17131789

Balzhan Savdenbekova, Aruzhan Sailau, Ayazhan Seidulayeva, Zhanar Bekissanova, Ardak Jumagaziyeva, Renata Nemkayeva

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引用次数: 0

Abstract

The development of antibacterial coatings for biomedical applications is crucial to prevent implant-associated infections (IAIs). In this study, we designed and evaluated a multilayer coating based on chitosan (CHI), polyacrylic acid (PAA), and triclosan (TCS) using the layer-by-layer (LbL) self-assembly technique. The successful incorporation of TCS was confirmed by Fourier-transform infrared (FTIR) spectroscopy. Surface roughness and topography were analyzed using atomic force microscopy (AFM) and scanning electron microscopy (SEM). Additionally, the pH-dependent behavior of PAA/CHI films was studied to assess its effect on TCS loading. According to disk diffusion assays, coatings assembled at pH 5 (PAA5/CHI5/TCS) exhibited the strongest antibacterial activity, with inhibition zones of 60.0 ± 0.0 mm for S. aureus and 33.67 ± 1.5 mm for E. coli. The long-term stability of the coatings was evaluated by measuring the antibacterial activity after 1, 10, 20, 30, and 40 days, with results confirming that antimicrobial properties and structural integrity were preserved over time. Furthermore, TCS release kinetics were assessed under physiological (pH 7.4) and acidic (pH 5.5) conditions, revealing enhanced release at pH 5.5. These findings highlight the potential of this multilayer system for biomedical applications requiring both stability and pH-responsive drug release.

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长效抗菌PAA/CHI/三氯生多层膜的设计与表征

生物医学应用抗菌涂层的开发对于预防种植体相关性感染（IAIs）至关重要。在这项研究中，我们设计并评估了基于壳聚糖（CHI）、聚丙烯酸（PAA）和三氯生（TCS）的多层涂层，采用逐层（LbL）自组装技术。傅里叶变换红外光谱（FTIR）证实了TCS的成功结合。采用原子力显微镜（AFM）和扫描电镜（SEM）对表面粗糙度和形貌进行了分析。此外，研究了PAA/CHI薄膜的ph依赖性行为，以评估其对TCS负载的影响。膜片扩散实验结果表明，pH为5 （PAA5/CHI5/TCS）的膜层对金黄色葡萄球菌的抑制范围为60.0±0.0 mm，对大肠杆菌的抑制范围为33.67±1.5 mm。通过在1、10、20、30和40天后测量涂层的抗菌活性来评估涂层的长期稳定性，结果证实随着时间的推移，涂层的抗菌性能和结构完整性保持不变。此外，在生理（pH 7.4）和酸性（pH 5.5）条件下评估TCS的释放动力学，发现pH 5.5时释放增强。这些发现突出了这种多层系统在生物医学应用中需要稳定性和ph反应性药物释放的潜力。

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来源期刊

Polymers POLYMER SCIENCE-

CiteScore

8.00

自引率

16.00%

发文量

4697

审稿时长

1.3 months

期刊介绍： Polymers (ISSN 2073-4360) is an international, open access journal of polymer science. It publishes research papers, short communications and review papers. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Polymers provides an interdisciplinary forum for publishing papers which advance the fields of (i) polymerization methods, (ii) theory, simulation, and modeling, (iii) understanding of new physical phenomena, (iv) advances in characterization techniques, and (v) harnessing of self-assembly and biological strategies for producing complex multifunctional structures.