Bacteriophages for periodontal pathogens control: A systematic review

IF 4.8 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-07-09 DOI:10.1016/j.jdent.2025.105960

Felipe Gomes Dallepiane , Malena Alejandro Coimbra Nogueira , Lucas Menezes dos Anjos , Gilberto De Souza Melo , Bruno Henriques , Gislaine Fongaro , Ariadne Cristiane Cabral Cruz

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引用次数: 0

Abstract

Objectives

Faced with the growing challenge of bacterial resistance to antibiotics, bacteriophages emerge as a promising alternative due to their specificity and ability to lyse specific bacteria. This systematic review aims to analyze the efficacy of bacteriophage therapy in controlling the growth of periodontal pathogen.

Data sources

The protocol was registered in PROSPERO (CRD42023479696). The review followed PRISMA guidelines, utilizing a PICOS framework. Studies were identified through searches in seven databases (Embase, LILACS, PubMed, SCOPUS, Web of Science, Google Scholar and ProQuest) up to November 2024.

Study selection

In vitro studies were evaluating the use of bacteriophages for this purpose were considered eligible. References were selected through a two-phase process. RoBDEMAT was used to assess the risk of bias. The certainty of the cumulative evidence was evaluated using the GRADE criteria.

Results

Eight studies were included, all demonstrating bacteriophages are effective in controlling periodontopathogenic bacteria growth. Four periodontal pathogen bacteria were analyzed, including Aggregatibacter actinomycetemcomitans (n = 3), Fusobacterium nucleatum (n = 3), Streptococcus gordonii (n = 2), and Porphyromonas gingivalis (n = 1). Eleven different types of bacteriophages were described, including: S1249 (n = 2), ΦSG005 (n = 2), JD-Fnp1 (n = 1), d-Fnp2 (n = 1), JD-Fnp3 (n = 1), JD-Fnp4 (n = 1), JD-Fnp5 (n = 1), FNU1 (n = 1), Aabφ01 (n = 1), Aabφ01–1 (n = 1), and Fnp02 (n = 1). The predominant method for evaluating bacterial growth was optical density measurement (n = 7) colony-forming unit (CFU) (n = 3).

Conclusion

Bacteriophage therapy effectively controls periodontal pathogenic bacteria in vitro, offering a possibility promising strategy for treating periodontitis, especially in antimicrobial-resistant cases. However, further research is needed to validate these findings in more complex models, such as multispecies biofilms and clinical settings.

Clinical significance

This study underscores the potential of bacteriophages as an innovative and targeted alternative for managing periodontal disease.

Abstract Image

查看原文本刊更多论文

控制牙周病原体的噬菌体：系统综述。

目的：面对细菌对抗生素耐药性日益增长的挑战，噬菌体由于其特异性和裂解特定细菌的能力而成为一种有希望的替代方案。本文旨在分析噬菌体治疗在控制牙周病原菌生长方面的疗效。数据来源：该协议在PROSPERO中注册（CRD42023479696）。审查遵循PRISMA指南，利用PICOS框架。截至2024年11月，通过在7个数据库（Embase、LILACS、PubMed、SCOPUS、Web of Science、b谷歌Scholar和ProQuest）中进行检索确定了研究。研究选择：评估噬菌体用于此目的的体外研究被认为是合格的。通过两个阶段的过程选择参考文献。使用RoBDEMAT评估偏倚风险。使用GRADE标准评估累积证据的确定性。结果：纳入8项研究，均证实噬菌体对牙周病病原菌的生长有控制作用。分析了4种牙周病原菌，包括放线菌聚集菌（n=3）、核梭菌（n=3）、戈登链球菌（n=2）和牙龈卟啉单胞菌（n=1）。描述了11种不同类型的噬菌体，包括：S1249 （n=2）、ΦSG005 （n=2）、JD-Fnp1 （n=1）、D-Fnp2 （n=1）、JD-Fnp3 （n=1）、JD-Fnp4 （n=1）、JD-Fnp5 （n=1）、FNU1 （n=1）、Aabφ01 （n=1）、Aabφ01-1 （n=1）和fnp2 （n=1）。评价细菌生长的主要方法是光密度测量（n=7）和菌落形成单位（CFU）（n=3）。结论：噬菌体治疗可有效控制牙周致病菌，为治疗牙周炎特别是耐药牙周炎提供了一种有前景的治疗策略。然而，需要进一步的研究来验证这些发现在更复杂的模型中，如多物种生物膜和临床环境。临床意义：这项研究强调了噬菌体作为治疗牙周病的创新和有针对性的替代方案的潜力。

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.