Growth properties of recombinant equine influenza viruses with different backbones generated by reverse genetics in embryonated chicken eggs.

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2025-07-12 DOI:10.1007/s00705-025-06368-5

Manabu Nemoto, Nanako Kawanishi, Yoshinori Kambayashi, Hiroshi Bannai, Takashi Yamanaka, Marie Garvey, Ann Cullinane, Seiya Yamayoshi, Yoshihiro Kawaoka, Koji Tsujimura

引用次数: 0

Abstract

Reverse genetics (RG) technology is useful for quickly updating influenza vaccine strains. A high-yield backbone (i.e., six segments other than hemagglutinin and neuraminidase) derived from A/Puerto Rico/8/1934 (PR8) has been developed to improve the growth of avian and human influenza viruses. However, for equine influenza virus (EIV), an EIV-derived backbone may have better growth properties due to more-natural segment combinations. We compared the growth properties in eggs between the PR8 backbone and an EIV backbone from A/equine/Ibaraki/1/2007, a vaccine strain in Japan. The results showed that the PR8 backbone was propagated more efficiently and is therefore more suitable for EIV vaccine production.

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反向遗传不同主干重组马流感病毒在鸡胚蛋中的生长特性。

反向遗传（RG）技术可用于快速更新流感疫苗株。已开发出源自A/Puerto Rico/8/1934 （PR8）的高产骨架（即血凝素和神经氨酸酶以外的六个片段），以促进禽流感病毒和人流感病毒的生长。然而，对于马流感病毒（EIV），由于更自然的片段组合，EIV衍生的主干可能具有更好的生长特性。我们比较了来自日本A/equine/Ibaraki/1/2007疫苗株的PR8脊椎骨和EIV脊椎骨在鸡蛋中的生长特性。结果表明，PR8主链扩增效率较高，更适合用于EIV疫苗的生产。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.