Genetic Characterization of a Linezolid- and Penicillin-Resistant Enterococcus hirae Isolate Co-Harboring poxtA and pbp5fm

IF 3 2区农林科学 Q2 INFECTIOUS DISEASES

Transboundary and Emerging Diseases Pub Date : 2025-07-13 DOI:10.1155/tbed/9275403

Jinhu Huang, Aijuan Li, Mengli Wang, Shushu Gu, He Hu, Xiaoming Wang, Liping Wang

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引用次数: 0

Abstract

Linezolid and penicillin are critical for treating multidrug resistant (MDR) Gram-positive infections, but the emergence of resistance to both seriously threatens public health. Here, we first report the cocarrying poxtA (oxazolidinone resistance) and pbp5fm (β-lactam resistance) genes by the plasmid in a strain of Enterococcus hirae HDC14-2 derived from porcine. The isolate also exhibits MDR phenotypes to phenicols, oxazolidinones, tetracyclines, β-lactams, aminoglycosides, macrolides, and lincosamides. Whole-genome sequencing (WGS) revealed these resistance genes, along with tet(L), tet(M), catA, erm(B), aac(6)-aph(2”), aadE, spw, lsa(E), lnu(B), sat4, and aphA3, were clustered in a novel MDR region flanked by IS1216 elements on plasmid pHDC14-2.133K. This IS1216-bounded MDR region formed translocatable units (TUs), including an IS1216-poxtA TU that was also identified on a secondary plasmid, pHDC14-2.27K. Functional assays demonstrated the excisability and mobility of these TUs, indicating its potential ability integration into other plasmids or chromosomes. Critically, electrotransformation confirmed the transfer of pHDC14-2.27K (poxtA-carrying) to Enterococcus faecalis JH2-2, with retained TU activity and minimal fitness cost. This study provides the evidence of colocalized poxtA and pbp5fm on plasmids in enterococci, highlighting their role in disseminating pan-resistance among bacteria. Although E. hirae is not an important pathogenic bacterium to humans and animals, but its potential risk to horizontally spread of these resistance genes important in medicine still cannot be ignored.

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一株含poxtA和pbp5fm的耐利奈唑胺和青霉素的hire肠球菌分离株的遗传特征

利奈唑胺和青霉素对于治疗耐多药革兰氏阳性感染至关重要，但对这两种药物的耐药性的出现严重威胁着公共卫生。本研究首次报道了一株猪源性霍乱肠球菌HDC14-2的质粒共携带poxtA （oxazolidinone耐药）和pbp5fm （β-内酰胺耐药）基因。该分离物对酚类、恶唑烷酮类、四环素类、β-内酰胺类、氨基糖苷类、大环内酯类和林肯胺类也表现出耐多药表型。全基因组测序（WGS）显示，这些耐药基因与tet(L)、tet(M)、catA、erm(B)、aac(6)-aph（2”）、aadE、spw、lsa(E)、lnu(B)、sat4和aphA3一起聚集在质粒pHDC14-2.133K上一个新的MDR区域，该区域两侧有IS1216元件。这个以is1216为界的MDR区域形成了可转位单元（translocable units， TU），包括一个IS1216-poxtA TU，该TU也在次级质粒pHDC14-2.27K上被鉴定出来。功能分析证明了这些tu的可切除性和可迁移性，表明其潜在的整合其他质粒或染色体的能力。关键的是，电转化证实了pHDC14-2.27K（携带poxta）向粪肠球菌JH2-2的转移，保留了TU活性和最小的适应度成本。本研究提供了poxtA和pbp5fm在肠球菌质粒上共定位的证据，突出了它们在细菌间传播泛耐药的作用。虽然鼠疫杆菌不是人类和动物的重要致病菌，但其对这些医学上重要的耐药基因水平传播的潜在风险仍然不容忽视。

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来源期刊

Transboundary and Emerging Diseases 农林科学-传染病学

CiteScore

8.90

自引率

9.30%

发文量

350

审稿时长

1 months

期刊介绍： Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.