Cover Feature: Synthetic Thermo-Sensitive Hydrogel Microspheres as Temperature Probes in an Optical Tweezers Setup (Chem. Methods 7/2025)

IF 6.1 Q1 CHEMISTRY, MULTIDISCIPLINARY

Chemistry methods : new approaches to solving problems in chemistry Pub Date : 2025-07-14 DOI:10.1002/cmtd.70015

Shahar Goren, Nicole Edelstein-Pardo, Ran Glinowiecki, Amit Sitt, Raya Sorkin, Ayelet Lesman

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Abstract

The Cover Feature shows poly-N-isopropyl-acrylamide (PNIPAAm) microspheres on a glass slide in water. PNIPAAm is a temperature-sensitive polymer that undergoes a volume transition between 20 and 45 °C. The spheres were fluorescently labelled and imaged with confocal fluorescence microscopy at 22 °C (top left) and at 45 °C (bottom right). In their Research Article (DOI: 10.1002/cmtd.202400040), R. Sorkin, A. Lesman and co-workers explain how they used the volume transition of PNIPAAm microspheres to measure the temperature close to a powerful optical tweezers laser beam. Measuring the temperature directly by using PNIPAAm helps to conduct optical tweezers experiments, especially with biological samples, and provides an additional control variable.

Abstract Image

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封面特征：合成热敏水凝胶微球作为光学镊子装置中的温度探头（化学）。方法7/2025)

封面特征显示了聚n -异丙基丙烯酰胺（PNIPAAm）微球在水中的玻璃载玻片上。PNIPAAm是一种温度敏感的聚合物，其体积在20 ~ 45°C之间发生转变。对球体进行荧光标记，并用共聚焦荧光显微镜在22°C（左上）和45°C（右下）下成像。在他们的研究文章（DOI: 10.1002/cmtd.202400040）中，R. Sorkin, a . Lesman及其同事解释了他们如何利用PNIPAAm微球的体积跃迁来测量接近强大光镊激光束的温度。使用PNIPAAm直接测量温度有助于进行光镊实验，特别是对生物样品，并提供了一个额外的控制变量。

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来源期刊

Chemistry methods : new approaches to solving problems in chemistry

CiteScore

7.30

自引率

0.00%

发文量