In situ diversity of the marine diatom genus Pseudo-nitzschia based on morphological characterisation

IF 2.6 3区地球科学 Q1 MARINE & FRESHWATER BIOLOGY

Estuarine Coastal and Shelf Science Pub Date : 2025-07-09 DOI:10.1016/j.ecss.2025.109433

J. Arapov , M. Bužančić , S. Skejić , J. Mandić , T. Bonačić , T. Tomašević , Ž. Ninčević Gladan

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Abstract

This study presents the first comprehensive analysis of the distribution, species composition, and morphology of the marine diatom genus Pseudo-nitzschia in four shellfish aquaculture areas along the Croatian coast. During the two-year period, Pseudo-nitzschia species were frequently occurring (96–100 %) across all areas, but their abundance remained generally low, except for two extreme bloom events in winter 2022. The abundance during the bloom reached 3.6 × 10⁶ cells L⁻¹ in Mali Ston Bay and 2.5 × 10⁶ cells L⁻¹ in Šibenik Bay, contributing up to 99 % of the phytoplankton community. A detailed species composition was analysed using Scanning Electron Microscopy in field samples. Eight Pseudo-nitzschia species were morphologically identified indicating the considerable diversity of this genus. Also high diversity was observed during bloom events. Multivariate analyses revealed significant spatial and seasonal variation in the Pseudo-nitzschia community structure. The Velebit Channel was characterised by higher frequency and contribution of P. calliantha and P. mannii, while in the other areas P. delicatissima complex, P. pseudodelicatissima/cuspidata, and P. galaxiae were more common and present throughout the year. Evident seasonality was observed for P. calliantha and P. subfraudulenta with winter-autumn annual peak associated with TIN and orthosilicates. Other species, such as P. pseudodelicatissima/cuspidata correspond to increase temperature and lower orthophosphates concentration, whereas the environmental factors mentioned exhibited the opposite effect on P. delicatisima complex and P. galaxiae.

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基于形态特征的海洋硅藻属伪硅藻的原位多样性

本文首次对克罗地亚沿海四个贝类养殖区的海洋硅藻属pseudonitzschia的分布、物种组成和形态进行了综合分析。在2年的时间里，伪nitzschia物种在所有地区频繁出现（96 - 100%），但除了2022年冬季的两次极端水华事件外，它们的丰度总体上保持在较低水平。藻华期间，马里斯顿湾的丰度达到3.6 × 106 cells L−1，Šibenik湾的丰度达到2.5 × 106 cells L−1，占浮游植物群落的99%。利用扫描电子显微镜对野外样品进行了详细的物种组成分析。在形态学上鉴定了8种伪尼茨奇亚，表明该属具有相当大的多样性。在开花期间也观察到较高的多样性。多变量分析表明，伪尼茨氏菌群落结构具有显著的空间和季节差异。Velebit海峡的特征是P. calliantha和P. mannii的频率和贡献较高，而在其他地区P. delicatissima complex， P. pseudodelicatissima/cuspidata和P. galaxae更常见，全年都有。花萼花和亚花萼花具有明显的季节性，其TIN和正硅酸盐的含量在冬秋季达到峰值。其他物种，如伪鱼/虎鱼，对应于温度升高和正磷酸盐浓度降低，而上述环境因素对鱼复合体和星系鱼的影响相反。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Estuarine Coastal and Shelf Science 地学-海洋学

CiteScore

5.60

自引率

7.10%

发文量

374

审稿时长

9 months

期刊介绍： Estuarine, Coastal and Shelf Science is an international multidisciplinary journal devoted to the analysis of saline water phenomena ranging from the outer edge of the continental shelf to the upper limits of the tidal zone. The journal provides a unique forum, unifying the multidisciplinary approaches to the study of the oceanography of estuaries, coastal zones, and continental shelf seas. It features original research papers, review papers and short communications treating such disciplines as zoology, botany, geology, sedimentology, physical oceanography.