New findings of ciliates Buxtonella sulcata in the faeces of cattle from northern regions of Russia and comparative analysis of Buxtonella species

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2025-07-10 DOI:10.1016/j.vprsr.2025.101314

O.A. Kornilova , M.A. Skazina , M.E. Belokon , I.V. Gusarov , V.V. Platonov , G.N. Machakhtyrov , V.A. Machakhtyrova , L.V. Chistyakova

{"title":"New findings of ciliates Buxtonella sulcata in the faeces of cattle from northern regions of Russia and comparative analysis of Buxtonella species","authors":"O.A. Kornilova , M.A. Skazina , M.E. Belokon , I.V. Gusarov , V.V. Platonov , G.N. Machakhtyrov , V.A. Machakhtyrova , L.V. Chistyakova","doi":"10.1016/j.vprsr.2025.101314","DOIUrl":null,"url":null,"abstract":"<div><div><em>Buxtonella sulcata</em> is a common ciliate that inhabits the intestines of cattle and can cause diarrhea and other clinical signs of infection. The distribution of <em>B. sulcata</em> in Russia has been practically unstudied, while the buxtonellosis it causes can have a negative impact on the health of cattle. This study aimed to morphologically and molecularly identify cysts and trophozoites of <em>B.sulcata</em>. This study provides data on the first finding of infection with <em>B. sulcata</em> ciliates in cattle from two northern regions of Russia. The structural features of <em>B. sulcata</em> were studied using immunofluorescence microscopy and silver impregnation methods. The phylogenetic position of the studied isolates was determined using sequences of the SSU and ITS regions. The results of our comprehensive comparative analysis revealed distinct features that enable the differentiation of species within the genus <em>Buxtonella</em>.</div></div>","PeriodicalId":23600,"journal":{"name":"Veterinary parasitology, regional studies and reports","volume":"63 ","pages":"Article 101314"},"PeriodicalIF":1.4000,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary parasitology, regional studies and reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405939025001224","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Buxtonella sulcata is a common ciliate that inhabits the intestines of cattle and can cause diarrhea and other clinical signs of infection. The distribution of B. sulcata in Russia has been practically unstudied, while the buxtonellosis it causes can have a negative impact on the health of cattle. This study aimed to morphologically and molecularly identify cysts and trophozoites of B.sulcata. This study provides data on the first finding of infection with B. sulcata ciliates in cattle from two northern regions of Russia. The structural features of B. sulcata were studied using immunofluorescence microscopy and silver impregnation methods. The phylogenetic position of the studied isolates was determined using sequences of the SSU and ITS regions. The results of our comprehensive comparative analysis revealed distinct features that enable the differentiation of species within the genus Buxtonella.

查看原文本刊更多论文

俄罗斯北部地区牛粪便中毛纤毛虫短布氏体的新发现及布氏体种类的比较分析

沟巴克通体是一种常见于牛肠内的纤毛虫，可引起腹泻和其他临床感染症状。事实上，人们尚未对短叶螺旋体在俄罗斯的分布进行研究，而它引起的布氏菌病可能对牛的健康产生负面影响。本研究旨在从形态学和分子结构上对南芥的包囊和滋养体进行鉴定。本研究提供了俄罗斯北部两个地区牛中首次发现的sulata B.纤毛虫感染的数据。采用免疫荧光显微镜和银浸渍法研究了南芥的结构特征。利用SSU区和ITS区序列确定所研究分离株的系统发育位置。我们的综合比较分析的结果揭示了明显的特征，使物种内的巴克斯通体的区分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).