Mai Guan Fu Kang tablet (MGFK) accelerates diabetic foot ulcer healing via antioxidant defense and inflammation suppression

IF 6.7 1区 医学 Q1 CHEMISTRY, MEDICINAL
Xiaowen Jiang , Ziyang Han , Yue Jin , Jie Liu , Yijia Zhao , Yujia Gu , Dapeng Zhou , Keqiang Li , Huiyuan Gao
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引用次数: 0

Abstract

Background

Diabetic foot ulcer (DFU), a severe complication of diabetes characterized by impaired healing and high morbidity, demand novel therapeutic strategies. Mai Guan Fu Kang tablets (MGFK) and combined therapy have been used in the clinical treatment of DFU, showing potential in DFU management, but their mechanisms remain unclear.

Purpose

This study aimed to elucidate the molecular mechanisms of MGFK in DFU, focusing on oxidative stress, inflammation, and vascular-neural repair.

Methods

Firstly, we performed a comprehensive pathological analysis of tissue samples obtained from patients with chronic diabetic wounds. Subsequently, we conducted in-depth investigations using DFU mouse model and diabetic lower limb ischemia rat model, as well as network pharmacology approaches. The efficacy and underlying mechanisms of MGFK were further elucidated through advanced analytical techniques, including liquid chromatography-mass spectrometry (LC-MS), molecular docking, qPCR, Western blotting, and immunohistochemistry (IHC).

Results

Clinical pathological analysis of tissue samples demonstrated significantly elevated levels of reactive oxygen species (ROS) and pro-inflammatory cytokines in patients with DFU. MGFK-treated DFU mice could significantly increase the wound healing rate, reduce inflammation and inhibit oxidative stress. In diabetic limb ischemia rat model, MGFK improved blood flow, reduced coagulation markers (FIB, PT), and restored sciatic nerve function, correlating with increased VEGF and NO levels. Network pharmacology and molecular docking highlighted key components suggesting that MGFK is related to inflammation-related pathways and targets. Finally, through the construction of LPS-induced Raw264.7 cell models and tert‑butyl hydroperoxide (TBHP)-induced L929 cell models, it was found that MGFK has significant anti-inflammatory and antioxidant stress effects in vitro. Finally, we formulated a compound prescription, whose anti-inflammatory effect in vitro is superior to that of MGFK.

Conclusions

These findings highlight the multi-target mechanism of MGFK, which integrates antioxidant, anti-inflammatory, and vascular repair properties. Specifically, MGFK promotes wound healing by restoring antioxidant capacity, inhibiting NF-κB-mediated inflammatory responses, and enhancing vascular function.

Abstract Image

麦管复康片通过抗氧化防御和炎症抑制促进糖尿病足溃疡愈合
背景:糖尿病足溃疡(DFU)是糖尿病的一种严重并发症,其特点是愈合不良且发病率高,需要新的治疗策略。麦管附康片(MGFK)及联合治疗已被应用于DFU的临床治疗,显示出治疗DFU的潜力,但其作用机制尚不清楚。目的本研究旨在阐明MGFK在DFU中的分子机制,重点关注氧化应激、炎症和血管神经修复。方法首先对慢性糖尿病创面组织标本进行全面病理分析。随后,我们采用DFU小鼠模型和糖尿病下肢缺血大鼠模型,以及网络药理学方法进行了深入研究。通过液相色谱-质谱(LC-MS)、分子对接、qPCR、Western blotting和免疫组织化学(IHC)等先进的分析技术,进一步阐明了MGFK的疗效和潜在机制。结果组织样本的临床病理分析显示,DFU患者的活性氧(ROS)和促炎细胞因子水平显著升高。mgfk处理DFU小鼠可显著提高创面愈合率,减轻炎症,抑制氧化应激。在糖尿病肢体缺血大鼠模型中,MGFK改善血流量,降低凝血标志物(FIB, PT),恢复坐骨神经功能,并与VEGF和NO水平升高相关。网络药理学和分子对接强调了关键成分,表明MGFK与炎症相关的途径和靶点有关。最后,通过构建lps诱导的Raw264.7细胞模型和TBHP诱导的L929细胞模型,发现MGFK在体外具有显著的抗炎和抗氧化应激作用。最后,我们配制了复方,其体外抗炎作用优于MGFK。结论MGFK具有抗氧化、抗炎、血管修复等多种作用机制。具体来说,MGFK通过恢复抗氧化能力、抑制NF-κ b介导的炎症反应和增强血管功能来促进伤口愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Phytomedicine
Phytomedicine 医学-药学
CiteScore
10.30
自引率
5.10%
发文量
670
审稿时长
91 days
期刊介绍: Phytomedicine is a therapy-oriented journal that publishes innovative studies on the efficacy, safety, quality, and mechanisms of action of specified plant extracts, phytopharmaceuticals, and their isolated constituents. This includes clinical, pharmacological, pharmacokinetic, and toxicological studies of herbal medicinal products, preparations, and purified compounds with defined and consistent quality, ensuring reproducible pharmacological activity. Founded in 1994, Phytomedicine aims to focus and stimulate research in this field and establish internationally accepted scientific standards for pharmacological studies, proof of clinical efficacy, and safety of phytomedicines.
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