Characterization and taxonomic clarification of a non-emetic, radiation-resistant Bacillus cereus strain mrbd isolated from a 60Co irradiator pool

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2025-07-08 DOI:10.1016/j.meegid.2025.105796

A.Y.K.M. Masud Rana , Safaiatul Islam , Abu Hena Mostofa Kamal , Issay Narumi

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Abstract

This study isolated a radiation-resistant Bacillus species from the pool water of a ⁶⁰Co gamma irradiator. The bacterium was motile, a spore-former, rod-shaped, Gram-positive, and capable of withstanding 5 kGy of gamma radiation. Based on 16S rRNA gene sequencing, MLST, and gyrB gene phylogeny, the isolate was classified within the B. cereus group. Comparative whole-genome analysis demonstrated a high level nucleotide identity with B. cereus AH820 (99.49 %), B. anthracis (98.59–98.81 %), and B. cereus type strain ATCC 14579 (91.70 %). Despite the chromosomal similarity to B. cereus AH820, the isolate lacked the emetic toxin-producing plasmid (PER_AH820) and phenotypic traits of B. anthracis, such as enterotoxin production and penicillin resistance. In silico DNA-DNA hybridization (DDH) assays showed 88.4 % genome similarity to B. anthracis, while showing a lower similarity (44.7 %) to B. cereus. Detection of non-B. anthracis ancestral “G” allele in the plcR gene further excluded its identification as B. anthracis. Whole-genome phylogenetic analysis clustered the isolate with B. cereus strains previously identified from the International Space Station (ISS) and pathogenic variants of B. cereus. The isolate was designated as B. cereus strain mrbd.

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从60Co辐照池中分离的一株非呕吐、耐辐射蜡样芽孢杆菌mrbd的鉴定和分类澄清。

本研究从60Co γ辐照器池水中分离出一种抗辐射芽孢杆菌。这种细菌是可运动的，孢子形成体，棒状，革兰氏阳性，能够承受5 kGy的伽马辐射。基于16S rRNA基因测序、MLST和gyrB基因系统发育，将该分离物归入蜡样芽孢杆菌属。比较全基因组分析显示，该菌株与蜡样芽孢杆菌AH820（99.49 %）、炭疽芽孢杆菌（98.59-98.81 %）和蜡样芽孢杆菌型菌株ATCC 14579（91.70 %）核苷酸高度同源。尽管与蜡样芽孢杆菌AH820染色体相似，但分离物缺乏吐毒质粒（PER_AH820）和炭疽芽孢杆菌的肠毒素产生和青霉素耐药性等表型性状。计算机DNA-DNA杂交（DDH）分析显示，与炭疽芽孢杆菌的基因组相似性为88.4 %，而与蜡样芽孢杆菌的相似性较低（44.7 %）。检测nonB。plcR基因中的G等位基因进一步排除了其为炭疽杆菌的鉴定。全基因组系统发育分析将该分离物与先前从国际空间站发现的蜡样芽孢杆菌菌株和蜡样芽孢杆菌的致病性变体聚集在一起。该分离物被鉴定为蜡样芽孢杆菌mrbd。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .