Advanced Microscopic Characterization and Antimicrobial Applications of Green-Synthesized Zinc Oxide Nanoparticles in Clinical Wound Healing Bandages.

IF 2.1 3区工程技术 Q2 ANATOMY & MORPHOLOGY

Microscopy Research and Technique Pub Date : 2025-07-11 DOI:10.1002/jemt.70028

D Malathy, G Kaladevi, B Subash, A Sahaya Raja, Sandhanasamy Devanesan, Munusamy Settu, Saurav Dixit, V Prathipa

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Abstract

The green synthesis of nanoparticles using plant extracts has garnered significant attention as a reliable and sustainable method for producing functional nanomaterials. Among these, zinc oxide (ZnO) nanoparticles are extensively studied for their potential biological applications. In this study, ZnO nanoparticles were synthesized through a green synthesis approach using an aqueous extract of Musa acuminata leaves and zinc acetate as precursors. The resulting nanoparticles were characterized using ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), x-ray diffraction analysis (XRD), energy-dispersive x-ray analysis (EDX), and scanning electron microscopy (SEM). The UV-Vis spectra revealed characteristic absorption peaks around 350 nm, attributed to the nanoparticles' large excitation binding energy at room temperature. FTIR analyses confirmed the formation of zinc oxide chemical bonds, while XRD results indicated a hexagonal wurtzite crystal structure. SEM analysis showed that the nanoparticles had a nearly cuboid shape, and EDX analysis confirmed their high purity. The minimum inhibitory concentrations (MIC) of the synthesized ZnO nanoparticles were evaluated against Staphylococcus aureus and Escherichia coli cultures. Cotton wound bandages impregnated with ZnO nanoparticles at concentrations near the calculated MIC demonstrated significant antibacterial activity in vitro. These antimicrobial bandages show potential for use in treating and protecting infection-prone wounds, such as diabetic or burn-related injuries.

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绿色合成氧化锌纳米颗粒的显微表征及其在临床创面愈合绷带中的抗菌应用。

利用植物提取物绿色合成纳米颗粒作为一种可靠和可持续的生产功能纳米材料的方法已引起广泛关注。其中氧化锌纳米颗粒因其潜在的生物学应用而受到广泛的研究。本研究以麝香叶水提物和乙酸锌为前体，采用绿色合成方法合成了ZnO纳米颗粒。利用紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FTIR）、x射线衍射分析（XRD）、能量色散x射线分析（EDX）和扫描电子显微镜（SEM）对纳米颗粒进行了表征。在室温下，纳米颗粒具有较大的激发结合能，紫外可见光谱显示出350 nm左右的特征吸收峰。FTIR分析证实了氧化锌化学键的形成，而XRD结果显示为六方纤锌矿晶体结构。SEM分析表明纳米颗粒呈近长方体形状，EDX分析证实其纯度高。测定了ZnO纳米颗粒对金黄色葡萄球菌和大肠杆菌的最低抑菌浓度（MIC）。氧化锌纳米颗粒浸渍棉质创面，其浓度接近计算的MIC，在体外表现出显著的抗菌活性。这些抗菌绷带显示出在治疗和保护易感染伤口（如糖尿病或烧伤相关损伤）方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microscopy Research and Technique 医学-解剖学与形态学

CiteScore

5.30

自引率

20.00%

发文量

233

审稿时长

4.7 months

期刊介绍： Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.