Osmaikon Lisboa Lobato, Tayná da Silva Nogueira, Tobias Emílio Tavares Lima, Felipe José da Costa Andrade, Marília Gabryelle Guimarães de Macedo, Rayane de Souza Pereira, Joilson Xavier, Mariene Ribeiro Amorim, Priscilla Paschoal Barbosa, Alex Sobrinho da Rocha, Silvokleio da Costa Silva, Luiz Carlos Junior Alcantara, William M de Souza, José Luiz Proenca-Modena, Érica Azevedo Costa, Adelino Soares Lima Neto, Lauro César Soares Feitosa, Maria do Socorro Pires E Cruz, Silvana Maria Medeiros de Sousa Silva, Silvia de Araújo França Baêta, Marcelo Adriano da Cunha E Silva Vieira, Sharon L Deem, Lilian Silva Catenacci
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引用次数: 0
Abstract
Background: The cycle of the Orthoflavivirus nilense (West Nile virus - WNV) involves birds and mosquitoes, while humans and equids serve as terminal hosts. In 2014, the first human case in Brazil was confirmed in Piauí State.
Objectives: To investigate the presence of WNV in birds, mosquitoes, and equids in municipalities of Piauí.
Methods: Collections were carried out following recommendations from the Ministry of Health of Brazil, in 11 municipalities (all with human cases or bird mortality), where biological samples were collected from birds, mosquitoes, and equids. The Viral RNA extraction was performed using a commercial kit, following the manufacturers' recommendations; samples were subjected to reverse transcription and polymerase chain reaction, with specific primers for WNV.
Findings: 2,706 samples were collected (636 birds, belonging to 99 species; 420 equids, and 1,650 mosquitoes, grouped into 346 pools, totaling 18 species. No collected sample yielded a positive result, corroborating with other studies showing the difficulty of molecular detection of WNV in healthy animals, which may explain the non-detection, in addition to the delayed diagnosis in humans.
Main conclusions: A local investigation involving suspected cases is still recommended in animals; however, in locations with late diagnosis in humans we suggest a serological survey of asymptomatic birds and equids.
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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