NGS-based transcriptome analysis of starry flounder (Platichthys stellatus) reveals key immune-related genes and host defense mechanisms against Streptococcus parauberis infection

IF 2.4 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-07-08 DOI:10.1016/j.dci.2025.105410

Min-Young Sohn , Ji-Min Jeong , Gyoungsik Kang , Kyung-Ho Kim , Ha-Jeong Son , HyeongJin Roh , Chan-Il Park

{"title":"NGS-based transcriptome analysis of starry flounder (Platichthys stellatus) reveals key immune-related genes and host defense mechanisms against Streptococcus parauberis infection","authors":"Min-Young Sohn , Ji-Min Jeong , Gyoungsik Kang , Kyung-Ho Kim , Ha-Jeong Son , HyeongJin Roh , Chan-Il Park","doi":"10.1016/j.dci.2025.105410","DOIUrl":null,"url":null,"abstract":"<div><div>The starry flounder (<em>Platichthys stellatus</em>) is a high-value aquaculture species in Korea, but its farming is hindered by <em>Streptococcus parauberis</em> infection. We used NGS-based de novo assembly to identify immunity-related genes and molecular markers in starry flounder.</div><div>Using de novo assembly, we analyzed transcriptomic changes in liver and leukocytes following infection. The liver exhibited greater transcriptional variability, indicating its role in systemic immune regulation, while the leukocytes focused on pathogen recognition. Transcriptomic profiling revealed a temporal shift in immune response: early activation of DNA damage repair pathways at 3 h post-infection indicated rapid host recognition and stress response, which later transitioned into immune modulation and energy conservation at 48 h, as evidenced by the downregulation of MAPK, TNF, and NF-κB signaling pathways. This pattern reflects a dynamic and regulated host defense strategy.</div><div>These findings provide foundational data for disease-resistant strain development, demonstrating the utility of NGS-based transcriptomics in identifying key immune genes in non-model fish species.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"170 ","pages":"Article 105410"},"PeriodicalIF":2.4000,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental and comparative immunology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0145305X25000990","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

The starry flounder (Platichthys stellatus) is a high-value aquaculture species in Korea, but its farming is hindered by Streptococcus parauberis infection. We used NGS-based de novo assembly to identify immunity-related genes and molecular markers in starry flounder.

Using de novo assembly, we analyzed transcriptomic changes in liver and leukocytes following infection. The liver exhibited greater transcriptional variability, indicating its role in systemic immune regulation, while the leukocytes focused on pathogen recognition. Transcriptomic profiling revealed a temporal shift in immune response: early activation of DNA damage repair pathways at 3 h post-infection indicated rapid host recognition and stress response, which later transitioned into immune modulation and energy conservation at 48 h, as evidenced by the downregulation of MAPK, TNF, and NF-κB signaling pathways. This pattern reflects a dynamic and regulated host defense strategy.

These findings provide foundational data for disease-resistant strain development, demonstrating the utility of NGS-based transcriptomics in identifying key immune genes in non-model fish species.

查看原文本刊更多论文

基于ngs的星形比目鱼（Platichthys stellatus）转录组分析揭示了副金黄色葡萄球菌感染的关键免疫相关基因和宿主防御机制。

星形比目鱼（Platichthys stellatus）在韩国是一种高价值的水产养殖品种，但其养殖受到副金黄色链球菌感染的阻碍。我们利用NGS-based de novo assembly对星形比目鱼免疫相关基因和分子标记进行了鉴定。使用de novo组装，我们分析了感染后肝脏和白细胞的转录组变化。肝脏表现出更大的转录变异性，表明其在全身免疫调节中的作用，而白细胞则专注于病原体识别。转录组学分析揭示了免疫反应的时间变化：感染后3小时DNA损伤修复途径的早期激活表明宿主快速识别和应激反应，随后在48小时转变为免疫调节和能量守恒，MAPK、TNF和NF-κB信号通路下调。这种模式反映了一种动态和规范的主机防御策略。这些发现为抗病菌株的发展提供了基础数据，证明了基于ngs的转录组学在鉴定非模式鱼类的关键免疫基因方面的实用性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.