ATG16L1 membrane recruitment in autophagy.

IF 6.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Critical Reviews in Biochemistry and Molecular Biology Pub Date : 2025-07-11 DOI:10.1080/10409238.2025.2521321

Fujing Wei, Nan Qin, Yunling Chen, Zhenzhen Liu, Xiaozhu Zhao, Xiaoying Yu, Ziling Feng, Yu Wang, Aimin Yang, Hongjuan Cui

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引用次数: 0

Abstract

Autophagy, a highly conserved catabolic pathway in eukaryotes, is essential for cellular survival during starvation and for maintaining cellular homeostasis. Central to autophagy is the de novo formation of double-membrane autophagosomes, which requires the orchestrated action of a set of autophagy-related (ATG) proteins. ATG16L1 is a core autophagy protein involved in distinct phases of autophagosome biogenesis, including membrane remodeling and the formation of phagophore-like membrane cups. It interacts with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex, which functions as an E3-like enzyme to catalyze LC3 lipidation. The membrane targeting of the ATG12-ATG5-ATG16L1 complex is crucial for regulating autophagy and preventing ectopic membrane engagement. In this review, we summarize and discuss the potential mechanisms underlying ATG16L1 membrane recruitment, focusing on its intrinsic membrane-binding properties and partner-mediated recruitment pathways. We critically explore how these multiple mechanisms collectively ensure the proper localization and function of ATG16L1, thereby regulating the initiation of autophagy, LC3 lipidation, and the sequestration of bacteria during xenophagy.

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ATG16L1膜在自噬中的募集。

自噬是真核生物中高度保守的分解代谢途径，对细胞在饥饿状态下的生存和维持细胞稳态至关重要。自噬的核心是双膜自噬体的重新形成，这需要一系列自噬相关（ATG）蛋白的协调作用。ATG16L1是一种核心自噬蛋白，参与自噬体生物发生的不同阶段，包括膜重塑和吞噬体样膜杯的形成。它与ATG12-ATG5偶联物相互作用形成ATG12-ATG5- atg16l1复合物，该复合物作为类似e3的酶催化LC3脂化。ATG12-ATG5-ATG16L1复合物的膜靶向对于调节自噬和防止异位膜接合至关重要。在这篇综述中，我们总结并讨论了ATG16L1膜募集的潜在机制，重点是其固有的膜结合特性和伴侣介导的募集途径。我们批判性地探讨了这些多重机制如何共同确保ATG16L1的正确定位和功能，从而调节自噬的启动、LC3脂化和异种吞噬过程中细菌的隔离。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Biochemistry and Molecular Biology 生物-生化与分子生物学

CiteScore

14.90

自引率

0.00%

发文量

期刊介绍： As the discipline of biochemistry and molecular biology have greatly advanced in the last quarter century, significant contributions have been made towards the advancement of general medicine, genetics, immunology, developmental biology, and biophysics. Investigators in a wide range of disciplines increasingly require an appreciation of the significance of current biochemical and molecular biology advances while, members of the biochemical and molecular biology community itself seek concise information on advances in areas remote from their own specialties. Critical Reviews in Biochemistry and Molecular Biology believes that well-written review articles prove an effective device for the integration and meaningful comprehension of vast, often contradictory, literature. Review articles also provide an opportunity for creative scholarship by synthesizing known facts, fruitful hypotheses, and new concepts. Accordingly, Critical Reviews in Biochemistry and Molecular Biology publishes high-quality reviews that organize, evaluate, and present the current status of high-impact, current issues in the area of biochemistry and molecular biology. Topics are selected on the advice of an advisory board of outstanding scientists, who also suggest authors of special competence. The topics chosen are sufficiently broad to interest a wide audience of readers, yet focused enough to be within the competence of a single author. Authors are chosen based on their activity in the field and their proven ability to produce a well-written publication.