Development of a time-resolved fluorescence immunochromatographic strip for gB antibody detection of PRV.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2025-07-11 DOI:10.1186/s12917-025-04913-7

Suzhen Yang, Yaning Sun, Yunrui Xing, Lu Fan, Yongliang Li, Yanli Shang, Shujun Chai, Yunchao Liu

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Abstract

Background: Pseudorabies (PR) is a highly contagious disease, and it causes significant economic losses to the global swine industry. Vaccination plays an important role in the prevention and control of pseudorabies virus (PRV). To evaluate vaccine efficacy, there is a need for a quick and straight forward method to monitor PRV-induced antibody levels in practice.

Results: A time-resolved fluorescence immunochromatographic (TRFIC) strip was developed for the serological detection of PRV gB antibodies in swine. Following systematic analysis and evaluation, the assay demonstrated a high degree of correlation with established reference method. The positive and negative coincidence rates between the TRFIC strip and ELISA were 96.8% and 94.2%, respectively. Furthermore, comprehensive analytical and comparative assessments revealed that the TRFIC strip exhibited no cross-reactivity with antibodies against other porcine pathogens.

Conclusion: Given its high specificity, sensitivity, and convenience, the TRFIC strip is suitable for on-site detection of PRV gB antibodies and can serve as a valuable tool for monitoring PRV immune status in animal populations.

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PRV gB抗体时间分辨荧光免疫层析试纸条的研制。

背景：伪狂犬是一种高度传染性疾病，给全球养猪业造成重大经济损失。疫苗接种在预防和控制伪狂犬病毒（PRV）中起着重要作用。为了评估疫苗效力，需要一种快速和直接的方法来监测prv诱导的抗体水平。结果：建立了一种时间分辨荧光免疫层析（TRFIC）条带，用于猪PRV gB抗体的血清学检测。经过系统的分析和评价，该方法与建立的参考方法高度相关。TRFIC条带与ELISA的阳性符合率为96.8%，阴性符合率为94.2%。此外，综合分析和比较评估表明，TRFIC条带与其他猪病原体抗体无交叉反应性。结论：TRFIC试条具有特异性高、灵敏度高、使用方便等特点，可用于PRV gB抗体的现场检测，可作为动物群体PRV免疫状态监测的重要工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.