Preparation of cellulose based hydrogel 3D SERS flexible substrate for the detection of urea in sweat

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-07-08 DOI:10.1016/j.saa.2025.126664

Yujun Zheng , Ziting Ye , Shuyan Ruan , Jingxin Chen , Lanjin Zhu , Youmei Liu , Yudong Lu , Zixing Yan

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Abstract

Au NRs/HA/CMC-PVA hydrogel membranes were successfully prepared by mixing sodium hyaluronate (HA) and carboxymethyl cellulose (CMC) solutions for immersion crosslinking on the basis of freeze-thaw crosslinking of polyvinyl alcohol (PVA) with gold nanorods (Au NRs). By adjusting the density of Au NRs and the concentrations of CMC and HA, more ‘hot spots’ could be generated to enhance the SERS signals, thus improving the sensitivity and homogeneity of the hydrogels (low detection limit of 10⁻⁹ mol/L Rhodamine 6G (R6G), RSD = 3.04 %). Meanwhile, the strong hydrogen bonds formed in the hydrogel network were utilized to increase the cross-linking degree of the hydrogels, which led to the improvement of the mechanical properties and flexural flexibility of the hydrogels (elongation at break of 308.07 % and tensile strength of 26.09 MPa). The hydrophilic groups of the Au NRs/HA/CMC-PVA hydrogel film were able to adsorb sweat and diffuse urea molecules from sweat into the hydrogel. It showed high sensitivity for the detection of urea, exhibiting a linear relationship at concentrations of 1000–1 mM, with a LOD = 7.83 × 10⁻⁵ M.

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用于汗液中尿素检测的纤维素基水凝胶三维SERS柔性底物的制备

在聚乙烯醇（PVA）与金纳米棒（Au NRs）冻融交联的基础上，将透明质酸钠（HA）与羧甲基纤维素（CMC）溶液混合进行浸没交联，成功制备了Au NRs/HA/CMC-PVA水凝胶膜。通过调整Au nmr的密度和CMC、HA的浓度，可以产生更多的“热点”，增强SERS信号，从而提高水凝胶的灵敏度和均匀性（低检出限为10 ~ 9 mol/L罗丹明6G (R6G), RSD = 3.04%）。同时，利用水凝胶网络中形成的强氢键，提高了水凝胶的交联度，提高了水凝胶的力学性能和弯曲柔韧性（断裂伸长率为308.07%，抗拉强度为26.09 MPa）。Au NRs/HA/CMC-PVA水凝胶膜的亲水性基团能够吸附汗液并将汗液中的尿素分子扩散到水凝胶中。该方法对尿素的检测具有较高的灵敏度，在浓度为1000-1 mM时呈线性关系，LOD = 7.83 × 10 - 5 M。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.