A label-free fluorescence polarization technology for the detection of food additives

IF 4.6 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-07-01 DOI:10.1016/j.saa.2025.126634

Shanshan Fan , Jilin Wang , Xueyi Wen , Yufei Feng , Fangfang Shao , Lin Xu , Siyu Liu , Wenxin Liu

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Abstract

A novel label-free fluorescence polarization (FP) assay utilizing bovine serum albumin nanoparticles (BSA NPs) in conjunction with manganese dioxide nanosheets (MnO₂ NS) has been developed for the simultaneous detection of glucose, choline and lactate. This method eliminated the need for antibodies, aptamers, or labeled dyes. The BSA NPs exhibit a weak FP signal, while the MnO₂ NS significantly amplificates the FP signal of the BSA NPs. The oxidizing properties of MnO₂ NS result in a marked reduction of the amplified FP signal of the BSA NPs-MnO₂ NS system with hydrogen peroxide (H₂O₂). Glucose, choline, and sodium lactate, which are prevalent food additives, can generate H₂O₂ through the action of their respective oxidases. Consequently, the BSA NPs-MnO₂ NS system was employed as a label-free platform for the FP detection of glucose, choline, and sodium lactate within the concentration ranges of 0–200 μM, 0–800 μM, and 0–200 μM, respectively.

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一种用于食品添加剂检测的无标记荧光偏振技术

利用牛血清白蛋白纳米颗粒（BSA NPs）结合二氧化锰纳米片（MnO2 NS），建立了一种新的无标记荧光偏振（FP）检测方法，用于同时检测葡萄糖、胆碱和乳酸。这种方法消除了对抗体、适体或标记染料的需要。BSA NPs表现出较弱的FP信号，而MnO2 NPs显著放大了BSA NPs的FP信号。MnO2 NS的氧化特性使BSA NPs-MnO2 NS体系的FP信号在过氧化氢（H2O2）作用下明显减弱。葡萄糖、胆碱和乳酸钠是常见的食品添加剂，它们通过各自氧化酶的作用产生H2O2。因此，采用BSA NPs-MnO2 NS系统作为无标记平台，分别在0 ~ 200 μM、0 ~ 800 μM和0 ~ 200 μM范围内对葡萄糖、胆碱和乳酸钠进行FP检测。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.