A novel C-type lectin gene with QPS and WND motifs in clam Meretrix meretrix

IF 2.4 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2025-07-09 DOI:10.1016/j.dci.2025.105416

Lu Zhang , Yu Tao , Bingxin Shi , Xin Jia , Qiang Xing , Ying Guo , Mengqiang Wang

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Abstract

C-type lectins (CTLs), as a class of classical calcium-dependent pattern recognition receptors (PRRs), plays an essential role in immune recognition and defense. In this study, a novel CTLs (MmCTL5) was identified from clam Meretrix meretrix, and the full-length cDNA of MmCTL5 encoded a 127-amino acids carbohydrate-recognition domain (CRD). The characteristic motifs of the Ca²⁺-binding site of MmCTL5 were QPS and WND. The typical double-loop structure of MmCTL5 contained three disulfide bonds at the bottom. Significant expression of MmCTL5 in hemocytes and hepatopancreas. After stimulation by Vibrio splendidus, the expression levels of MmCTL5 increased by 6.2-fold at 12 h in hemocytes and 9.4-fold at 3 h in hepatopancreas, respectively. The recombinant MmCTL5 protein (rMmCTL5) could agglutinate two kinds of pathogenic Vibrio parahemolyticus. And the rMmCTL5 could bind to a variety of carbohydrates, including four kinds of monosaccharides and two kinds of disaccharides. These results suggested that MmCTL5, as a novel CTL, took an essential part in innate immune response of M. meretrix.

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蛤蚌中具有QPS和WND基序的c型凝集素基因

c型凝集素（C-type lecectin, ctl）是一类经典的钙依赖性模式识别受体（calcium-dependent pattern recognition receptor, PRRs），在免疫识别和防御中发挥着重要作用。本研究从蛤蜊Meretrix Meretrix中鉴定出一个新的ctl (MmCTL5)，其全长cDNA编码一个127个氨基酸的碳水化合物识别结构域（CRD）。MmCTL5 Ca2+结合位点的特征基序为QPS和WND。MmCTL5典型的双环结构在底部含有3个二硫键。MmCTL5在血细胞和肝胰腺中显著表达。脾弧菌刺激后，MmCTL5在血细胞中的表达量在12 h时增加6.2倍，在肝胰脏中的表达量在3 h时分别增加9.4倍。重组MmCTL5蛋白（rMmCTL5）可凝集两种致病性副溶血性弧菌。rMmCTL5可以与多种碳水化合物结合，包括4种单糖和2种双糖。这些结果表明，MmCTL5作为一种新型CTL，在mermetrix的先天免疫应答中起着重要作用。

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.