Separation-free cholesterol detection using an enzyme-doped hydrogel opto-microfluidic hybrid chip via colorimetry and light extinction spectroscopy

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-07-09 DOI:10.1016/j.talanta.2025.128570

Quoc-Thinh Dinh , Pei-Yun Yu , Srishti Sen , Wen-Chien Chuang , Wei-Yu Chen , Cheng-Yang Liu

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Abstract

Cholesterol levels serve as a critical biomarker for the diagnosis of cardiovascular diseases. However, conventional detection methods are often time-consuming and reliant on specialized laboratory equipment. In this study, we present a novel microfluidic lab-on-a-chip platform designed for the rapid and accurate detection of cholesterol, employing an enzyme-doped agarose hydrogel combined with colorimetric analysis. The chip incorporates sensing zones of varying lengths (4 mm, 5 mm, and 6 mm), enabling multiple optical measurements from a single sample. By integrating optical absorbance with RGB imaging, the system achieves high sensitivity and specificity, with a detection limit as low as 0.0065 mM and a sensitivity of 0.411 absorbance/mM. The chip design features microlenses and optical fibers to enhance light transmission and promote smooth fluid flow. Requiring only 10 μL of sample, the platform is both minimally invasive and highly efficient. Validation experiments using serum and whole blood samples demonstrate excellent recovery rates (95.8 %–110.6 %) and high precision (relative standard deviation, RSD <3.54 %). This portable, cost-effective device offers a reliable solution for routine cholesterol monitoring and early cardiovascular risk assessment.

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酶掺杂水凝胶光微流控混合芯片的比色法和消光光谱无分离胆固醇检测

胆固醇水平是诊断心血管疾病的重要生物标志物。然而，传统的检测方法往往耗时且依赖于专门的实验室设备。在这项研究中，我们提出了一种新的微流控芯片实验室平台，设计用于快速准确地检测胆固醇，采用酶掺杂琼脂糖水凝胶结合比色分析。该芯片集成了不同长度的传感区（4mm， 5mm和6mm），能够从单个样品进行多次光学测量。该系统将光学吸光度与RGB成像相结合，具有较高的灵敏度和特异性，检出限低至0.0065 mM，灵敏度为0.411吸光度/mM。芯片设计采用微透镜和光纤来增强光的传输，促进流体的流畅流动。该平台仅需要10 μL的样品，具有微创和高效的特点。采用血清和全血样品进行验证实验，回收率为95.8% ~ 110.6%，精密度高（相对标准偏差，RSD < 3.54%）。这种便携、经济实惠的设备为常规胆固醇监测和早期心血管风险评估提供了可靠的解决方案。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.