Marker assisted introgression of blast resistance genes into Heritage Red Rice Landrace “Zag” of Western Himalayan Kashmir

IF 2.8 3区农林科学 Q2 PLANT SCIENCES

Physiological and Molecular Plant Pathology Pub Date : 2025-07-07 DOI:10.1016/j.pmpp.2025.102820

Musharib Gull , Asif B. Shikari , Gazala H. Khan , Parvaze A. Sofi , Nakeeb-Un- Nisa , M. Ashraf Ahanger , Basu Sudhakar Reddy , Aravind Jukanti , S.M. Zargar , F.A. Mohiddin , Shabir H. Wani , Suhail Ashraf , Mahandiya Iqbal , Shahid Mushtaq , Najeeb Ul Rehman Sofi

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引用次数: 0

Abstract

Pigmented rices are popular and are grown in specific ecological niches in various Indian states. The most prominent among them is red rice which is known for its distinctive flavour, texture and micronutrients (Fe & Zn). Zag is a cultivated red rice landrace from Jammu and Kashmir known for having comparatively higher levels of iron and zinc. However, the area under this cultivar is shrinking because it is highly susceptible to blast disease caused by Magnophortheoryzae, where losses are often as high as 70 % and complete yield loss may occur. To address this, in the present study a three-gene donor line, DHMAS70Q164-1b harbouring resistance genes Pi54, Pi1, and Pita, was used to incorporate the three genes into the genetic background of red the rice cultivar Zag using a marker-assisted backcross breeding programme (MABC). Foreground selection was carried out using molecular markers Pi54MAS, RM224 and YL 155/87 to select for genes Pi54, Pi1 and Pita respectively. In the BC₁F₁ 15 plants were screened for the genes Pi54, Pi1 and Pita and two of these plants K16-32-2 and K16-32-6 were found to be heterozygous for all three genes. The three-gene BC₁F₁ plants were selfed to produce a BC₁F₂generation. Based on plant and grain type 100 BC₁F₂ were selected and subjected to selection for identification of homozygotes for the three genes. Six BC₁F₂ plants out of 100 were found to be homozygotes for all the three genes, two were positive for two genes (Pi1+Pita) and two were positive for the Pita gene. These 10-backcross derived blast resistant lines were subjected to background analysis using a total of 67 genome wide polymorphic markers including KASP SNP genotyping with 51 SNPs. The introgressed lines were evaluated for agronomic traits and cooking quality as well as grain Zn and Fe content. The promising introgressed lines namelyK16-32-2-20, K16-32-2-22, K16-32-6-51 and K16-32-6-81 recorded a background genome recovery of 69, 86, 84 and 80 % respectively. The derived lines expressed resistance to diagnostic isolates under controlled conditions and showed resistance under natural field conditions. The backcross derived lines possess cooking quality traits and Fe & Zn content equal to those of the recurrent parent Zag. The lines are proposed for multi-location testing after seed multiplication and can be recommended as genetically improved resistant versions of the red rice var. Zag.

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标记辅助将抗稻瘟病基因导入西喜马拉雅克什米尔地区红稻“Zag”

色素水稻很受欢迎，在印度各个邦的特定生态位中种植。其中最突出的是红米，它以其独特的风味、质地和微量营养素(铁；锌)。Zag是一种来自查谟和克什米尔的红稻，以铁和锌含量相对较高而闻名。然而，该品种的面积正在缩小，因为它极易受到由magnnophortheoryzae引起的稻瘟病的影响，其损失往往高达70%，并可能发生完全的产量损失。为了解决这一问题，本研究采用标记辅助回交育种计划（MABC），利用含有抗性基因Pi54、Pi1和Pita的三基因供体DHMAS70Q164-1b，将这三个基因整合到红稻品种Zag的遗传背景中。利用分子标记Pi54MAS、RM224和YL 155/87分别选择Pi54、Pi1和Pita基因进行前景选择。在BC1F1中，共筛选到15株Pi54、Pi1和Pita基因，其中2株K16-32-2和K16-32-6均为这3个基因的杂合子。三基因BC1F1植株自交产生bc1f2代。根据植物和籽粒类型，选择100个BC1F2进行3个基因的纯合子鉴定。100株BC1F2株中6株为3个基因的纯合子，2株为2个基因（Pi1+Pita）阳性，2株为Pita基因阳性。利用67个全基因组多态性标记对这些10回交衍生的稻瘟病抗性品系进行背景分析，其中包括51个SNP的KASP SNP基因分型。对这些品系的农艺性状、蒸煮品质和籽粒锌、铁含量进行了评价。有潜力的渐渗品系k16 -32-2-20、K16-32-2-22、K16-32-6-51和K16-32-6-81的背景基因组回收率分别为69%、86%、84%和80%。衍生系在控制条件下对诊断分离株表现出抗性，在自然田间条件下表现出抗性。回交衍生系具有蒸煮品质性状和铁；锌的含量等于双亲Zag的含量。建议在种子繁殖后对这些品系进行多地点测试，并可推荐作为红水稻品种Zag的遗传改良抗性版本。

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来源期刊

Physiological and Molecular Plant Pathology 生物-植物科学

CiteScore

4.30

自引率

7.40%

发文量

130

审稿时长

38 days

期刊介绍： Physiological and Molecular Plant Pathology provides an International forum for original research papers, reviews, and commentaries on all aspects of the molecular biology, biochemistry, physiology, histology and cytology, genetics and evolution of plant-microbe interactions. Papers on all kinds of infective pathogen, including viruses, prokaryotes, fungi, and nematodes, as well as mutualistic organisms such as Rhizobium and mycorrhyzal fungi, are acceptable as long as they have a bearing on the interaction between pathogen and plant.