Genetic epidemiology of Plasmodium falciparum chloroquine resistance in coastal, North and Far North areas of Cameroon.

Abstract

Background: Unravelling the population genetic structure and dynamics of drug-resistant Plasmodium falciparum (Pf) strains is essential to adapt control strategies. Here, genetic and evolutionary patterns of P. falciparum chloroquine resistance transporter (Pfcrt) in Cameroon were analysed.

Methods: Blood samples from P. falciparum-infected individuals living in a coastal area (Douala) and North and Far North areas (Maroua, Mayo-Oulo, Pette) were PCR-amplified and genotyped for the Pfcrt gene. Single nucleotide polymorphism analysis, haplotype network, neutral evolution and genetic differentiation patterns of Pfcrt sequences were also performed.

Results: The wild-type CVMNK was dominant (97.1%) in the coastal area, while the resistance genotype CVIET (50-61.9%) was predominant in the North and Far North areas. The mutation 72S was significantly more often reported in asymptomatic infections (p=0.004) and submicroscopic parasitaemia cases (p=0.002). A signature of positive selection or population expansion was identified in Pfcrt sequences from Douala. The Pf populations from coastal, North and Far North areas were genetically differentiated.

Conclusions: This study suggests a return to chloroquine susceptibility geographically restricted in the coastal region. The high circulation of the genotype CVIET in North and Far North areas calls for investigations of factors contributing to the persistence of chloroquine-resistant strains.