Effect of titanium nanoparticles on cytotoxicity and expression of matrix metalloproteinase-8 in human periodontal ligament fibroblasts - An in vitro study.
{"title":"Effect of titanium nanoparticles on cytotoxicity and expression of matrix metalloproteinase-8 in human periodontal ligament fibroblasts - An <i>in vitro</i> study.","authors":"Saurav Shankar Das, Nandini N Krishnamurthy, Mohana Kumar, Riolla Sanchia Dsouza","doi":"10.4103/jisp.jisp_266_24","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims and objectives: </strong>Initial research suggested a potential link between titanium particles and peri-implantitis. Titanium's susceptibility to fretting, a process that generates microscopic debris, and its passive layer's breakdown in inflammatory environments contribute to the release of titanium ions. However, the full impact of these particles on implant health remains unclear. Therefore, the aim of the study is to evaluate the effect of different concentrations of titanium nanoparticles on the cytotoxicity and matrix metalloproteinase-8 expression in human periodontal ligament fibroblasts (HPDLfs).</p><p><strong>Materials and methods: </strong>To investigate the effects of titanium (Ti) on HPDLfs, a standard Ti solution was diluted to create various concentrations (0.001, 0.1, 1, and 100 ppm). HPDLFs were cultured in media containing these Ti concentrations, and their viability was assessed after 24 and 48 h. Based on these cytotoxicity results, optimal Ti concentrations (0.01, 0.1, and 10 ppm) were selected for further analysis. The expression of matrix metalloproteinase-8 (MMP-8) was measured using quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) to evaluate the impact of Ti on HPDLfs.</p><p><strong>Results: </strong>Exposure to titanium (Ti) reduced the viability of HPDLFs in a concentration-and time-dependent manner. At 100 ppm, more than 50% cell death was observed. qRT-PCR indicated a significant up-regulation of MMP-8 expression. However, ELISA analysis did not reveal any significant changes in MMP-8 levels.</p><p><strong>Conclusion: </strong>Within the study's limitations, the cytotoxic and immunological responses of HPDLFs to titanium (Ti) are directly linked to the concentration and duration of exposure. Although no significant differences in MMP-8 release were observed across varying TiO2 concentrations, a decrease in cell viability was associated with higher TiO2 levels.</p>","PeriodicalId":15890,"journal":{"name":"Journal of Indian Society of Periodontology","volume":"29 1","pages":"63-72"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12237240/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Indian Society of Periodontology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jisp.jisp_266_24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/6/10 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Dentistry","Score":null,"Total":0}
引用次数: 0
Abstract
Aims and objectives: Initial research suggested a potential link between titanium particles and peri-implantitis. Titanium's susceptibility to fretting, a process that generates microscopic debris, and its passive layer's breakdown in inflammatory environments contribute to the release of titanium ions. However, the full impact of these particles on implant health remains unclear. Therefore, the aim of the study is to evaluate the effect of different concentrations of titanium nanoparticles on the cytotoxicity and matrix metalloproteinase-8 expression in human periodontal ligament fibroblasts (HPDLfs).
Materials and methods: To investigate the effects of titanium (Ti) on HPDLfs, a standard Ti solution was diluted to create various concentrations (0.001, 0.1, 1, and 100 ppm). HPDLFs were cultured in media containing these Ti concentrations, and their viability was assessed after 24 and 48 h. Based on these cytotoxicity results, optimal Ti concentrations (0.01, 0.1, and 10 ppm) were selected for further analysis. The expression of matrix metalloproteinase-8 (MMP-8) was measured using quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) to evaluate the impact of Ti on HPDLfs.
Results: Exposure to titanium (Ti) reduced the viability of HPDLFs in a concentration-and time-dependent manner. At 100 ppm, more than 50% cell death was observed. qRT-PCR indicated a significant up-regulation of MMP-8 expression. However, ELISA analysis did not reveal any significant changes in MMP-8 levels.
Conclusion: Within the study's limitations, the cytotoxic and immunological responses of HPDLFs to titanium (Ti) are directly linked to the concentration and duration of exposure. Although no significant differences in MMP-8 release were observed across varying TiO2 concentrations, a decrease in cell viability was associated with higher TiO2 levels.
期刊介绍：
The Journal of Indian Society of Periodontology publishes original scientific articles to support practice , education and research in the dental specialty of periodontology and oral implantology. Journal of Indian Society of Periodontology (JISP), is the official publication of the Society and is managed and brought out by the Editor of the society. The journal is published Bimonthly with special issues being brought out for specific occasions. The ISP had a bulletin as its publication for a large number of years and was enhanced as a Journal a few years ago
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
