Wound Dressing Potential of Bacterial Cellulose Produced by Acetobacter tropicalis NBRC 16470 Strain Isolated from Rotten Fruits.

IF 2.2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current pharmaceutical biotechnology Pub Date : 2025-07-08 DOI:10.2174/0113892010361594250703060501

Halil Bal

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引用次数: 0

Abstract

Background: Bacterial cellulose, which is used in many fields from biomedicine to electronics, is promising as an alternative wound dressing instead of traditional gauze in wound treatment.

Objectives: The objective of this study was to evaluate the potential use of cellulose produced by acetic acid bacteria isolated from rotten fruits as a wound dressing.

Methods: In our study, rotten fruit samples were incubated in Hestrin-Schramm (HS) Broth medium. Then, a loopful of the pellicle-forming samples was taken and inoculated onto Hestrin- Schramm (HS) agar using the streak culture method and bacteria were isolated. Identification of bacteria was performed using the BLAST program after 16S rRNA sequence analysis. Physicochemical properties and morphological characterization of bacterial cellulose produced by static culture were examined using Fourier transform infrared (FTIR) and scanning electron microscopy (SEM), respectively, and the swelling ratio was investigated. Antibiotic susceptibilities of bacterial cellulose membranes impregnated with different concentrations of gentamicin (50 μg/mL, 100 μg/mL, 200 μg/mL) against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922 were determined by the disk diffusion method.

Results: The bacteria isolated from rotten fruits were identified as Acetobacter tropicalis NBRC 16470. The structure of cellulose produced by static culture was confirmed by a peak at 3,240 cm-1 in FTIR analysis and fibril structures in SEM analysis. Bacterial cellulose had a swelling ratio of 27.37± 2 .99 fold. The zone diameters formed by bacterial cellulose disk (50 μg/mL gentamicin) and gentamicin (10 μg) disk against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922 were almost the same.

Conclusion: The production of bacterial cellulose, which has the potential to be used as a wound dressing from rotten fruits, is important in terms of recycling and low cost.

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腐果中分离的热带醋酸杆菌NBRC 16470产细菌纤维素的创面敷料潜力

背景：细菌纤维素作为一种替代传统纱布的创面敷料，被广泛应用于从生物医学到电子等多个领域。目的：本研究的目的是评估从腐烂水果中分离的乙酸细菌产生的纤维素作为伤口敷料的潜在用途。方法：在本研究中，腐烂的水果样品在hstrin - schramm （HS）肉汤培养基中培养。然后取一圈成膜样品，用条纹培养法接种于hstrin - Schramm （HS）琼脂上，分离细菌。16S rRNA序列分析后，使用BLAST程序进行细菌鉴定。采用傅里叶变换红外（FTIR）和扫描电镜（SEM）对静态培养细菌纤维素的理化性质和形态特征进行了研究，并对其溶胀率进行了研究。采用纸片扩散法测定不同浓度庆大霉素（50 μg/mL、100 μg/mL、200 μg/mL）浸染细菌纤维素膜对金黄色葡萄球菌ATCC 29213和大肠杆菌ATCC 25922的敏感性。结果：从腐烂水果中分离到的细菌鉴定为热带醋酸杆菌NBRC 16470。通过FTIR分析和SEM分析，在3240 cm-1处有一个峰，证实了静态培养纤维素的结构。细菌纤维素的溶胀率为27.37±2.99倍。细菌纤维素片（50 μg/mL庆大霉素）和庆大霉素片（10 μg/mL）对金黄色葡萄球菌ATCC 29213和大肠杆菌ATCC 25922的作用区直径基本相同。结论：利用腐烂水果生产细菌纤维素具有可循环利用和低成本的优点，具有创面敷料的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current pharmaceutical biotechnology 医学-生化与分子生物学

CiteScore

5.60

自引率

3.60%

发文量

203

审稿时长

6 months

期刊介绍： Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal includes timely in-depth reviews, original research articles and letters written by leaders in the field, covering a range of current topics in scientific areas of Pharmaceutical Biotechnology. Invited and unsolicited review articles are welcome. The journal encourages contributions describing research at the interface of drug discovery and pharmacological applications, involving in vitro investigations and pre-clinical or clinical studies. Scientific areas within the scope of the journal include pharmaceutical chemistry, biochemistry and genetics, molecular and cellular biology, and polymer and materials sciences as they relate to pharmaceutical science and biotechnology. In addition, the journal also considers comprehensive studies and research advances pertaining food chemistry with pharmaceutical implication. Areas of interest include: DNA/protein engineering and processing Synthetic biotechnology Omics (genomics, proteomics, metabolomics and systems biology) Therapeutic biotechnology (gene therapy, peptide inhibitors, enzymes) Drug delivery and targeting Nanobiotechnology Molecular pharmaceutics and molecular pharmacology Analytical biotechnology (biosensing, advanced technology for detection of bioanalytes) Pharmacokinetics and pharmacodynamics Applied Microbiology Bioinformatics (computational biopharmaceutics and modeling) Environmental biotechnology Regenerative medicine (stem cells, tissue engineering and biomaterials) Translational immunology (cell therapies, antibody engineering, xenotransplantation) Industrial bioprocesses for drug production and development Biosafety Biotech ethics Special Issues devoted to crucial topics, providing the latest comprehensive information on cutting-edge areas of research and technological advances, are welcome. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.