Topical Hepatocyte Growth Factor Accelerates Wound Healing and Inhibits Scarring in Experimental Corneal Injury.

IF 2.1 3区医学 Q2 OPHTHALMOLOGY

Cornea Pub Date : 2025-07-10 DOI:10.1097/ICO.0000000000003932

Elsayed Elbasiony, Sharad K Mittal, Vinay K Pulimamidi, Tomi Luan, Susan Orr, Reza Dana, Sunil K Chauhan

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引用次数: 0

Abstract

Purpose: The aim of this study was to compare the rates of healing, scar formation, and regression of preexisting scars using topical murine hepatocyte growth factor (mHGF), recombinant human deleted HGF (dHGF), murine nerve growth factor (mNGF), and phosphate-buffered saline (PBS).

Methods: Mechanical corneal epithelial and stromal injury was induced in C57BL/6 mice. Five groups of mice were studied in each of phase I (wound healing and scar formation) and phase II (regression of preexisting scars). Each group received topical 0.1% mHGF, 0.1% dHGF, 0.2% dHGF, 0.1% mNGF, or PBS. In phase I, animals were dosed 4 times/day (QID) on days 0 to 7. In phase II, a corneal injury was induced, and no treatment was administered for 10 days to allow scar formation. Mice with scars were then dosed QID on days 11 to 21. Ocular assessments included slit-lamp photography (phase I and II) and fluorescein staining (phase I).

Results: In phase I, eyes treated with mHGF exhibited significantly faster epithelial healing and decreased scar formation compared with both mNGF-treated and PBS-treated eyes 7 days after treatment. Consistent with that seen in mHGF-treated eyes, 0.1% and 0.2% dHGF-treated eyes each showed significantly faster epithelial healing compared with PBS-treated eyes. Scars formed in dHGF-treated eyes were also significantly smaller than PBS-treated eyes 7 days after treatment. In phase II, both mHGF-treated and dHGF-treated eyes showed a significant decrease in the size of preexisting scars compared with PBS-treated eyes.

Conclusions: Topical HGF significantly accelerates corneal wound healing, reduces scar formation, and can reverse preexisting scars.

Abstract Image

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外用肝细胞生长因子促进实验性角膜损伤创面愈合并抑制瘢痕形成。

目的：本研究的目的是比较外用小鼠肝细胞生长因子（mHGF）、重组人缺失HGF （dHGF）、小鼠神经生长因子（mNGF）和磷酸盐缓冲盐水（PBS）的愈合率、疤痕形成率和先前存在的疤痕的消退率。方法：对C57BL/6小鼠进行机械角膜上皮和角膜间质损伤。五组小鼠分别在第一阶段（伤口愈合和疤痕形成）和第二阶段（先前存在的疤痕消退）进行研究。各组外用0.1% mHGF、0.1% dHGF、0.2% dHGF、0.1% mNGF或PBS。在第一阶段，动物在第0 ~ 7天给药4次/天（QID）。在第二阶段，诱导角膜损伤，10天内不进行任何治疗以允许瘢痕形成。然后在第11天至第21天给疤痕小鼠服用QID。眼部评估包括裂隙灯摄影（I期和II期）和荧光素染色（I期）。结果：在I期试验中，与mngf治疗和pbs治疗的眼睛相比，mHGF治疗的眼睛在治疗7天后表现出明显更快的上皮愈合和减少疤痕形成。与mhgf处理的眼睛一致，0.1%和0.2% dhgf处理的眼睛与pbs处理的眼睛相比，上皮愈合明显更快。治疗7天后，dhgf治疗组的疤痕也明显小于pbs治疗组。在II期，与pbs治疗的眼睛相比，mhgf治疗和dhgf治疗的眼睛都显示出先前存在的疤痕大小显著减少。结论：外用HGF可显著加速角膜创面愈合，减少瘢痕形成，并可逆转原有瘢痕。

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来源期刊

Cornea 医学-眼科学

CiteScore

5.20

自引率

10.70%

发文量

354

审稿时长

3-6 weeks

期刊介绍： For corneal specialists and for all general ophthalmologists with an interest in this exciting subspecialty, Cornea brings together the latest clinical and basic research on the cornea and the anterior segment of the eye. Each volume is peer-reviewed by Cornea''s board of world-renowned experts and fully indexed in archival format. Your subscription brings you the latest developments in your field and a growing library of valuable professional references. Sponsored by The Cornea Society which was founded as the Castroviejo Cornea Society in 1975.