Structural characterization and osteogenic potential of polysaccharide purified from Sanghuangporus vaninii

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-07-05 DOI:10.1016/j.carbpol.2025.124018

Jiyu Song , Qing Xue , Haoran Wang , Ruijia Jin , Xiao Liu , Lanzhou Li , Yang Liu , Di Wang , Min Hu

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Abstract

Sanghuangporus vaninii (S. vaninii) is a fungus of considerable medicinal properties. Nevertheless, the specific effects of polysaccharides derived from S. vaninii on bone tissues and their underlying mechanisms remain underexplored. In this research, we purified polysaccharide (SVP-a2) from S. vaninii and systematically explored its structural composition and the anti-osteoporotic characteristics via promoting bone formation. SVP-a2 is a novel neutral polysaccharide exhibiting a molecular weight of 23.593 kDa, predominantly constituted by →6)-α-Galp-(1→. The substitutions are observed at specific C-2 positions, involving the α-Manp-(1 → or α-Manp-(1 → 2)-α-Fucp-(1→. In the dexamethasone-induced osteoporosis (OP) mice model, SVP-a2 significantly restored the bone microstructure, enhanced bone density and trabecular connectivity, and markedly promoted the osteogenic differentiation in the bone tissue. Through the combined analysis of serum metabolomics, bone tissue proteomics and biological assays, SVP-a2 was found to modulate bone metabolism by regulating mineral absorption. SVP-a2 activated the fibroblast growth factor 2/Wnt signaling pathway, inhibited glycogen synthase kinase-3β phosphorylation, promoted osteogenic differentiation, enhanced bone formation, and thereby exerted an anti-OP effect. This research furnishes a therapeutic approach for using SVP-a2 as a functional constituent in the clinical adjunctive therapy of OP, and presents an innovative theoretical foundation for the development of polysaccharide-based bone metabolism regulator.

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桑黄茯苓多糖的结构表征及成骨潜力

桑黄孢是一种具有重要药用价值的真菌。然而，从葡萄球菌中提取的多糖对骨组织的具体作用及其潜在机制仍未得到充分探讨。在本研究中，我们从缬草中纯化了多糖（SVP-a2），并系统地探讨了其结构组成及其促进骨形成的抗骨质疏松特性。SVP-a2是一种新型中性多糖，分子量为23.593 kDa，主要由→6)-α- galp -(1→组成。在特定的C-2位置观察到取代，包括α-Manp-(1→或α-Manp-(1→2)-α- fucp -（1→）。在地塞米松所致骨质疏松症（OP）小鼠模型中，SVP-a2显著恢复骨微结构，增强骨密度和骨小梁连系，显著促进骨组织成骨分化。通过血清代谢组学、骨组织蛋白质组学和生物学分析，发现SVP-a2通过调节矿物质吸收来调节骨代谢。SVP-a2激活成纤维细胞生长因子2/Wnt信号通路，抑制糖原合成酶激酶3β磷酸化，促进成骨分化，促进骨形成，从而发挥抗op作用。本研究为SVP-a2作为功能性成分用于OP的临床辅助治疗提供了治疗途径，为开发基于多糖的骨代谢调节剂提供了创新的理论基础。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.