Characterization of polysaccharides fractions from Trillium tschonoskii Maxim. and assessment of their antioxidant and immunomodulatory activities

IF 6.5 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications Pub Date : 2025-07-03 DOI:10.1016/j.carpta.2025.100935

Jing Chen , Yongheng Zhao , Jiao Hu , Zhihang Ye , Chunmei Nan , Pingfei Li , Xincai Hao

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Abstract

The present work aimed to explore different Trillium tschonoskii Maxim. polysaccharide (TTMPs) fractions derived by ethanol precipitation on the physicochemical properties and biological activities, four new fractions (TTMP-40, TTMP-50, TTMP-60 and TTMP-70) were obtained. TTMPs contained varying proportions of arabinose, glucosamine hydrochloride, galactose, glucose, xylose and galacturonic acid, and exhibited different total sugar content, uronic acid content and protein content. All fractions also showed different molecular weight and microstructure. TTMP-70 displayed the stronger antioxidant activity than that of TTMP-40, TTMP-50 and TTMP-60 in vitro. Furthermore, the immunomodulatory effects of TTMP-70 were mediated through reduction of intracellular ROS, which in turn inhibited H₂O₂-induced apoptosis and promoted CD8+ T cell proliferation. These results indicate that TTMP-70 has the potential to be a potent natural antioxidant with clinical and industrial applications.

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龙头草多糖组分的研究。并对其抗氧化和免疫调节活性进行评价

本研究的目的是探讨不同种类的三叶草。对乙醇沉淀法提取的多糖（TTMPs）的理化性质和生物活性进行了研究，得到了TTMP-40、TTMP-50、TTMP-60和TTMP-70四个新组分。TTMPs含有不同比例的阿拉伯糖、盐酸氨基葡萄糖、半乳糖、葡萄糖、木糖和半乳糖醛酸，总糖含量、醛酸含量和蛋白质含量也不同。各馏分也表现出不同的分子量和微观结构。体外实验表明，TTMP-70的抗氧化活性高于TTMP-40、TTMP-50和TTMP-60。此外，TTMP-70的免疫调节作用是通过减少细胞内ROS介导的，从而抑制h2o2诱导的细胞凋亡，促进CD8+ T细胞增殖。这些结果表明，TTMP-70有潜力成为一种具有临床和工业应用价值的强效天然抗氧化剂。

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来源期刊

Carbohydrate Polymer Technologies and Applications

CiteScore

8.70

自引率

0.00%

发文量